{"product_id":"biolegend-144701","title":"Biolegend, 144701, Purified anti-mouse Ly49H Antibody, 50μg","description":"\u003cp\u003eLy49H, also known as Cmv1 and Klra8, is a 28.3 kD protein.  It is expressed on NK cells (but not on NKT cells) from C57BL\/6 mice (but not Balb\/c or DBA\/2 mice).  Expression of Ly49H has been associated with resistance to MCMV infection in C57BL\/6 mice.  Modulation of Ly49H expression by its antibody made MCMV-resistant C57BL\/6 mice susceptible to infection.  Unlike Ly49A, Ly49H lacks the ITIM (immunoreceptor tyrosine-based inhibitory motif) but has ITAM (immunoreceptor tyrosine-based activation motif), which is associated with DAP-12 (KARAP, killer cell activating motif).\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: C1498-Ly49A\/H transfectant\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IP, Block - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats of this clone) include: immunoprecipation1, and in vitro2 and in vivo3 blocking activity. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 144717 \u0026amp; 144718).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Guseva NV, et al. 2010. Eur. J. Immunol. 40:2618. (IP) Orr MT, et al. 2009. J. Exp. Med. 206:807. (Block) Rodriguez M, et al. 2004. J. Immunol. 173:6312. (Block)\u003cbr\u003e\nProduct Citations: Potempa M, et al. 2022. J Immunol. 208:1742. PubMed Niogret C, et al. 2019. Nat Commun. 10:1444. PubMed Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed Goh W, et al. 2020. Cell Rep. 33:108285. PubMed Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed\u003cbr\u003e\nRRID: AB_2561549 (BioLegend Cat. No. 144702)\u003cbr\u003e\nStructure: 28.3 kD\u003cbr\u003e\nDistribution: NK cells but not NKT cells\u003cbr\u003e\nFunction: Interacts with m157, a cytomegalovirus-encoded protein\u003cbr\u003e\nLigand\/Receptor: m157\u003cbr\u003e\nCell Type: NK cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nAntigen References: 1. Gosselin P, et al. 1999. J. Leuko. Bio. 66:165. 2. Lee SH, et al. 2001. Nat. Genet. 28:42. 3. Daniels KA, et al. 2001. J. Exp. Med. 194:29.\u003cbr\u003e\nGene ID: 16639\u003cbr\u003e\nUniProt: View information about Ly49H on UniProt.org\u003cbr\u003e\nClone: 3D10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Klra8, Cmv1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862390853801,"sku":"144701","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-144701","provider":"Iright","version":"1.0","type":"link"}