{"product_id":"biolegend-145211","title":"Biolegend, 145211, PE\/Cyanine7 anti-mouse CD304 (Neuropilin-1) Antibody, 25μg","description":"\u003cp\u003eCD304, also known as neuropilin-1, is a 140 kD type I transmembrane protein. Its extracellular region contains two CUB, two FV\/FVIII, and one MAM domain. It is expressed by natural regulatory T cells (nTreg), a subset of invariant natural killer T cells (iNKT), endothelial cells, and neurons. Neuropilin-1 stabilizes the interaction between Tregs and dendritic cells, contributes to the recruitment of tumor-infiltrating Tregs in response to tumor-derived VEGF, and influences the process of angiogenesis and axon guidance. The ligands of CD304 are VEGF165 and semaphorin-3A.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Extracellular region of mouse CD304\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed Verhagen J, et al. 2014. PLoS One. 9e:108023. (FC) PubMed\u003cbr\u003e\nProduct Citations: Que W, et al. 2022. Sci Adv. 8:eabo4413. PubMed Bhattacharjee A, et al. 2021. Immunity. 54(8):1745-1757.e7. PubMed Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed Watson MJ, et al. 2021. Nature. 591:645. PubMed Beidaq A, et al. 2016. J Immunol. 197: 1567 - 1576. PubMed Ouyang S, et al. 2019. J Immunol. 202:1441. PubMed\u003cbr\u003e\nRRID: AB_2562359 (BioLegend Cat. No. 145211) AB_2562360 (BioLegend Cat. No. 145212)\u003cbr\u003e\nStructure: Type I transmembrane protein, 140 kD, 2 complement binding domains (CUB), 2 coagulation factor V\/VIII homology domains (FV\/FVIII), one meprin, A5, receptor tyrosine phosphatase domain (MAM)\u003cbr\u003e\nDistribution: Natural regulatory T cells (nTreg), subset of invariant natural killer T cells (iNKT), endothelial cells, neurons\u003cbr\u003e\nFunction: Angiogenesis, axon guidance, modulation of the immune response\u003cbr\u003e\nLigand\/Receptor: VEGF165, semaphorin-3A\u003cbr\u003e\nCell Type: Dendritic cells, Endothelial cells, Neurons, NKT cells, Tregs\u003cbr\u003e\nBiology Area: Angiogenesis, Cancer Biomarkers, Cardiovascular Biology, Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Synaptic Biology\u003cbr\u003e\nMolecular Family: Adaptor Proteins, Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Yadav M, et al. 2012. J. Exp. Med. 209:1713. 2. Weiss JM, et al. 2012. J. Exp. Med. 209:1723. 3. Hansen W, et al. 2012. J. Exp. Med. 209:2001. 4. Milpied P, et al. 2011. Blood 118:2993.\u003cbr\u003e\nGene ID: 18186\u003cbr\u003e\nUniProt: View information about CD304 on UniProt.org\u003cbr\u003e\nClone: 3E12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: NRP-1, VEGFR165 receptor\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864372105385,"sku":"145211","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-145211","provider":"Iright","version":"1.0","type":"link"}