{"product_id":"biolegend-145510","title":"Biolegend, 145510, Biotin anti-mouse CD185 (CXCR5) Antibody, 100μg","description":"\u003cp\u003eCD185 is also known as CXCR5. It is the receptor for chemokine CXCL13\/BLC, which is chemotactic for B cells.  CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow.  It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches.  Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: mCXCR5-transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.\u003cbr\u003e\nProduct Citations: Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed Yeh CH, et al. 2022. Immunity. 55:272. PubMed Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed Kim Y, et al. 2015. PLoS One. 10:120294. PubMed LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed Biram A, et al. 2020. Cell Rep. 30:1910. PubMed Kato Y, et al. 2020. Immunity. 53(3):548-563. PubMed Yang K, et al. 2018. J Virol. 92:e00428. PubMed Biram A, et al. 2020. Bio Protoc. e3562:10. PubMed Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed Zeiträg J, et al. 2021. Eur J Immunol. 51:408. PubMed Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed Reimer D, et al. 2020. Cell Reports. 32(6):108030. PubMed Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed\u003cbr\u003e\nRRID: AB_2562125 (BioLegend Cat. No. 145509) AB_2562126 (BioLegend Cat. No. 145510)\u003cbr\u003e\nStructure: 42 kD, seven transmembrane protein\u003cbr\u003e\nDistribution: B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain\u003cbr\u003e\nFunction: B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma\u003cbr\u003e\nLigand\/Receptor: BLC\/CXCL13\u003cbr\u003e\nCell Type: B cells, T cells, Tfh\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532. 2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381. 3. Forster R, et al. 1994. Blood 84:830. 4. Forster R, et al. 1996. Cell 87:1037. 5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831.\u003cbr\u003e\nGene ID: 12145\u003cbr\u003e\nUniProt: View information about CD185 on UniProt.org\u003cbr\u003e\nClone: L138D7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BLR1, GPCR6, MDR-15\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866116706473,"sku":"145510","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-145510","provider":"Iright","version":"1.0","type":"link"}