{"product_id":"biolegend-146009","title":"Biolegend, 146009, PerCP\/Cyanine5.5 anti-Asialo-GM1 Antibody, 25μg","description":"\u003cp\u003eGM1 is a ganglioside, a type of glycosphingolipid with a single sialic acid group. Asialo-GM1 is a GM1 derivative without a sialic acid group. It is expressed on NK cells, basophils, monocytes\/macrophages, and T cells. It is particularly expressed on very early thymocytes, but the expression decreases as the cells mature and become Thy-1 + . The highest expression is detected on neuronal tissues. This molecule has been shown to be involved in microbial pathogenesis. Antibodies specific for Asialo-GM1 are elevated in dementia, lupus, and Guillain-Barré syndrome.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse, Rat\u003cbr\u003e\nAntibody Type: Polyclonal\u003cbr\u003e\nHost Species: Rabbit\u003cbr\u003e\nImmunogen: Asialo-GM1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: This antibody recognizes asialo-GM1. It does not react with other glycolipids, such as GM1 and Asialo-GM2.This antibody can partially block IL-12 induced IFN-γ production but does not affect other systemic action of IL-12. This product may contain other non-IgG subtypes.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Naiki M, et al. 1974. J. Immunol. 113:84. Kasai M, et al. 1981. Nature. 291:334. (Depletion) Nishikado H, et al. 2011. J. Immunol. 186:5766. (Depletion) Zhou G, et al. 2013. Eur. J. Immunol. 43:929. (Depletion)\u003cbr\u003e\nRRID: AB_2728173 (BioLegend Cat. No. 146010)\u003cbr\u003e\nStructure: Asialo-GM1 is a GM1 derivative without a sialic acid group, with the structure: βDGalp(1-3)βDGalpNAc(1-4)βDGalp(1-4)βDGlcp(1-1)Cer\u003cbr\u003e\nDistribution: NK cells, basophils, splenic T lymphocytes, highest concentrations on neurons\u003cbr\u003e\nFunction: Supports cellular identification, neuronal plasticity, and repair mechanisms\u003cbr\u003e\nInteraction: Facilitates cellular recognition and cell-to-cell communication\u003cbr\u003e\nLigand\/Receptor: Ligand for cholera toxin\u003cbr\u003e\nCell Type: Basophils, Neurons, NK cells, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction\u003cbr\u003e\nAntigen References: 1. Stein-Douglas K, et al. 1979. J. Exp. Med. 143:822. 2. Kasai M, et al. 1980. Eur. J. Immunol. 10:175. 3. Young WW Jr, et al. 1980. J. Immunol. 124:199. 4. Bansal AS, et al. 1994. J. Clin. Path. 47:300.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nClone: Poly21460\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Ganglioside, GM1, ganglio-N-tetraosylceramide\u003cbr\u003e\nIsotype: Rabbit Polyclonal IgG\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866465849513,"sku":"146009","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-146009","provider":"Iright","version":"1.0","type":"link"}