{"product_id":"biolegend-146805","title":"Biolegend, 146805, Alexa Fluor® 647 anti-mouse CD301b (MGL2) Antibody, 25μg","description":"\u003cp\u003eMouse CD301, also known as macrophage galactose-type C-type lectin, has two homologue genes, CD301a (MGL1) and CD301b (MGL2), while there is only one MGL in human and rat.  Mouse CD301a and CD301b are ~42 kD type II transmembrane glycoproteins containing a cytoplasmic domain, a transmembrane domain, a neck domain, and a carbohydrate recognition domain (CRD) within each molecule. CD301a is mainly expressed on a subset of macrophages and immature dendritic cells (DCs). CD301b is mainly found on conventional DCs. Although CD301a and CD301b share high amino acid sequence homology (92% for the intact sequence and 80% for the CRD), they display different carbohydrate specificities. CD301a was found to be highly specific for Lewis X and Lewis A structures, whereas CD301b, more similar to human MGL, recognizes N-actetylgalactosamine (GalNAc) and galactose, including the O-linked Tn-antigen, TF-antigen, and core 2.  So far, CD301a has been reported to be involved in recognition and  endocytosis of glycoproteins with terminal Gal\/GalNAc moieties. This contributes to defense against tumor cell metastasis, tissue remodeling, and clearance of apoptotic cells in embryos. CD301b is involved in the internalization of soluble polyacrylamide polymers (PAA) with α-GalNAc residues (GalNAc-PAA) in bone marrow derived DCs.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Purified and recombinant mouse MGL2\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nProduct Citations: Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed\u003cbr\u003e\nRRID: AB_2563387 (BioLegend Cat. No. 146805) AB_2563388 (BioLegend Cat. No. 146806)\u003cbr\u003e\nStructure: 42 kD type II transmembrane glycoproteins, contains a cytoplasmic domain, a transmembrane domain, a neck domain, and a carbohydrate recognition domain (CRD) within each molecule\u003cbr\u003e\nDistribution: Dermal dendritic cells\u003cbr\u003e\nFunction: Cell adhesion, cell-cell signaling, glycoprotein turnover, inflammation, tissue remodeling\u003cbr\u003e\nInteraction: Carbohydrate determinants, GP envelope glycoprotein on Marburg and Ebola viruses\u003cbr\u003e\nCell Type: Dendritic cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Denda-Nagai K, et al. 2010. J. Biol. Chem. 285:19193. 2. Westcott D, et al. 2009. J. Exp. Med. 206:3143. 3. Singh SK, et al. 2009. Mol. Immunol. 46:1240. 4. Sakakura M, et al. 2008. J. Biol. Chem. 283:33665. 5. Tsuiji M, et al. 2002. J. Biol. Chem. 277:28892.\u003cbr\u003e\nGene ID: 216864\u003cbr\u003e\nUniProt: View information about CD301b on UniProt.org\u003cbr\u003e\nClone: URA-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MGL, M-ASGP-BP, Macrophage galactose-type C-type lectin\u003cbr\u003e\nIsotype: Rat IgG2a, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864080175273,"sku":"146805","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-146805","provider":"Iright","version":"1.0","type":"link"}