{"product_id":"biolegend-147606","title":"Biolegend, 147606, APC anti-mouse CD100 (SEMA4D) Antibody, 100μg","description":"\u003cp\u003eCD100, also known as Semaphorin-4D (SEMA4D), is a 150 kD, type I transmembrane protein, with one sema domain and one C2-type Ig domain. It is found as a homodimer on the cell surface. CD100 is expressed in the brain, kidney, heart, the majority of hematopoietic cells, and some malignant cells. CD100 is a potent inducer of angiogenesis and inhibitor of bone formation, and plays a role in lymphocyte activation, antibody production, dendritic cell maturation, and axon guidance. Its ligands are CD72 and Plexin-B1. CD100 is cleaved from the membrane of activated T and B cells, producing a soluble form.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse CD100-Fc fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications for the relevant formats include: immunoprecipitation from cell lysates2, capture antibody function in a sandwich ELISA assay2, and immunohistochemical staining of frozen brain sections4.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kumanogoh A, et al. 2000. Immunity. 13:621. (FC) Wang X, et al. 2001. Blood. 97:3498. (FC, IP, ELISA) Kumanogoh A, et al. 2002. J. Immunol. 169:1175. (FC) Moreau-Fauvarque C, et al. 2003 J. Neurosci. 23:9229. (IHC)\u003cbr\u003e\nProduct Citations: James OJ, et al. 2021. Nat Commun. 12:4290. PubMed\u003cbr\u003e\nRRID: AB_2563615 (BioLegend Cat. No. 147605)\u003cbr\u003e\nStructure: Type I transmembrane protein, one sema domain, one C2-type immunoglobulin domain, homodimer, 150 kD.\u003cbr\u003e\nDistribution: Highly expressed on most hematopoietic cells, is also found in the brain, kidney, and heart. Overexpressed by some malignant cell types.\u003cbr\u003e\nFunction: Potent inducer of angiogenesis, inhibitor of bone formation, plays a role in lymphocyte activation, antibody production and dendritic cell maturation. In neurons, regulates dendrite branching and axon guidance.\u003cbr\u003e\nLigand\/Receptor: CD72, Plexin-B1.\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Neutrophils, Tregs\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Immunology, Neuroscience, Signal Transduction, Synaptic Biology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell. 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.\u003cbr\u003e\nGene ID: 20354\u003cbr\u003e\nUniProt: View information about CD100 on UniProt.org\u003cbr\u003e\nClone: BMA-12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Neuroimmune semaphorin 4D, Sema4D, M-Sema G, Semaphorin-C-like 2, Semaphorin-J, Semaj, Semcl2, Coll-4, Semacl2\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862914977961,"sku":"147606","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-147606","provider":"Iright","version":"1.0","type":"link"}