{"product_id":"biolegend-147901","title":"Biolegend, 147901, Ultra-LEAF™ Purified anti-mouse CD300a Antibody, 100μg","description":"\u003cp\u003eCD300 receptors are a group of type I transmembrane proteins, which belong to the Ig superfamily. In mice, nine members have been reported with different nomenclature systems, such as myeloid-associated Ig-like receptor (MAIR), leukocyte mono-Ig-like receptor (LMIR), DC-derived Ig-like receptor (DIgR), and CMRF-35-like molecule (CLM). The CD300 molecules act as either activating or inhibitory receptors through interactions with adaptor molecules, such as PAD12, DAP10, and FcRγ. CD300a is an inhibitory receptor that is mainly expressed on peripheral eosinophils, mast cells, neutrophils, dendritic cells, macrophages, and some B cells. Cross-linking CD300a by an antibody can induce phosphorylation of tyrosine residues in the cytoplasmic domain, which results in recruitment of phosphatases SHIP, SHP1 and SHP2 and inhibition of cell activation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: MAIR-I transfected\u003cbr\u003e\nFormulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.\u003cbr\u003e\nEndotoxin Level: Less than 0.01 EU\/µg of the protein (\u0026lt; 0.001 ng\/µg of the protein) as determined by the LAL test.\u003cbr\u003e\nPreparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg\/mL and 3 mg\/mL. Older lots may have also been bottled at 1 mg\/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.\u003cbr\u003e\nApplication: FC - Quality tested IP, Activ - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for relevant formats) include: immunoprecipitation and induction of cytokine production by peritoneal macrophages.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Nakahashi-Oda C, et al. 2012. J. Exp. Med. 209:8. (FC, Block)\u003cbr\u003e\nRRID: AB_2563668 (BioLegend Cat. No. 147901)\u003cbr\u003e\nStructure: Ig superfamily, three immunoreceptor tyrosine-based inhibitory motifs (ITIMs), one non-canonical ITIM\u003cbr\u003e\nDistribution: Peripheral eosinophils, mast cells, neutrophils, dendritic cells, macrophages, and some B cells\u003cbr\u003e\nFunction: Acts as an inhibitory receptor\u003cbr\u003e\nInteraction: DAP12, DAP10, FcRγ\u003cbr\u003e\nLigand\/Receptor: Phosphatidylserine\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Eosinophils, Macrophages, Mast cells, Neutrophils\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Yotsumoto K, et al. 2003. J. Exp. Med. 198:223. 2. Martinez-Barriocanal A, et al. 2010. J. Biol. Chem. 285:41781. 3. Borrego F. 2013. Blood. 121:1951. 4. Lankry D, et al. 2013. Eur. J. Immunol. 43:2151.\u003cbr\u003e\nGene ID: 217303\u003cbr\u003e\nUniProt: View information about CD300a on UniProt.org\u003cbr\u003e\nClone: TX41\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD300a, MAIR-I, LMIR-I, CLM-8, CMRF-35H, IRp60\u003cbr\u003e\nIsotype: Rat IgG2a, λ\u003cbr\u003e\nQ: Do you guarantee that your antibodies are totally pathogen free?\u003cbr\u003e\nA: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?\u003cbr\u003e\nA: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?\u003cbr\u003e\nA: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.\u003cbr\u003e\nQ: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?\u003cbr\u003e\nA: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864058810537,"sku":"147901","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-147901","provider":"Iright","version":"1.0","type":"link"}