{"product_id":"biolegend-148106","title":"Biolegend, 148106, APC anti-mouse CD285 (TLR5) Antibody, 100μg","description":"\u003cp\u003eCD285, also known as TLR5, is a 98 kD pattern recognition receptor that binds to bacterial flagellin. It is expressed on dendritic cells, classical monocytes and mucosal epithelial cells and is involved in activation of TLR5 induced NF-κB mediated activation of inflammatory-related target genes. Mutations in the TLR5 gene have been linked with susceptibility to systemic lupus erythematosus and Legionnaire’s disease.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: TLR5-transfected Ba\/F3 cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications for the relevant formats include: immunoprecipitation of TLR5 from cell lysates1,2.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Shibata T, et al. Int Immunol. 24:613. (IP, FC) Huh JW, et al. 2014. Proc. Natl. Acad. Sci. USA. 111:7072. (IP)\u003cbr\u003e\nProduct Citations: Lee GR, et al. 2021. JCI Insight. 6:. PubMed\u003cbr\u003e\nRRID: AB_2890695 (BioLegend Cat. No. 148105) AB_2890695 (BioLegend Cat. No. 148106)\u003cbr\u003e\nStructure: Type 1 transmembrane protein, 98 kD\u003cbr\u003e\nDistribution: Neutrophils, CD11b, Ly6C, classical monocytes, dendritic cells, mucosal epithelium\u003cbr\u003e\nInteraction: Interacts with MyD88 and TRAF6 leading to NF-κB activation and production of cytokines.\u003cbr\u003e\nLigand\/Receptor: Flagellin\u003cbr\u003e\nCell Type: Dendritic cells, Monocytes, Neutrophils\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. López-Yglesias AH, et al. 2014. J. Immunol. 192:1587. 2. Burdelya L, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E1857. 3. Carapau D, et al. 2013. Infect. Immun. 81:4350. 4. Kinnebrew M, et al. 2012. Immunity. 36:276. 5. Sebastiani G, et al. 2000. Genomics. 64:230.\u003cbr\u003e\nGene ID: 53791\u003cbr\u003e\nUniProt: View information about CD285 on UniProt.org\u003cbr\u003e\nClone: ACT5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Toll-like receptor 5, TIL3, SLEB1, MELIOS\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862529986729,"sku":"148106","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-148106","provider":"Iright","version":"1.0","type":"link"}