{"product_id":"biolegend-148702","title":"Biolegend, 148702, Purified anti-mouse P2X7R Antibody, 100μg","description":"\u003cp\u003eP2X7R, also known as P2X7 receptor, belongs to the family of ligand-gated ion channel receptors. It is expressed on T cells, B cells, macrophages, and microglia. The receptor opens in the presence of extracellular ATP or NAD, leading to intracellular calcium mobilization. P2X7R activation requires higher concentrations of ATP compared to other P2X receptors. Longer stimulation results in larger pores, allowing passage of larger molecules. Activation of these molecules also leads to mitochondrial and cytoskeletal changes as well as IL-1β maturation and release. Ligation of P2X7 receptor can lead to membrane blebbing and cell death.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Murine colon mast cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality testedIP, IHC, WB - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications for the relevant formats include: immunoprecipitation1, Western blotting1, immunohistochemistry1, in vivo inhibition of intestinal inflammation and mast cell activation1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kurashima Y, et al. 2012. Nat. Commun. 3:1034. (FC, IP, WB, IHC, FA)\u003cbr\u003e\nProduct Citations: Sluyter R, et al. 2023. Int J Mol Sci. 24:. PubMed Yan J, et al. 2020. Cancer Immunol Res. 8:356. PubMed Guan F, et al. 2021. Infect Immun. 89: . PubMed Islam J, et al. 2022. Front Immunol. 13:766919. PubMed\u003cbr\u003e\nRRID: AB_2564122 (BioLegend Cat. No. 148702)\u003cbr\u003e\nStructure: Ligand-gated ion channel, trimer - two transmembrane regions and one cytosolic region.\u003cbr\u003e\nDistribution: Immune cells, including mast cells, microglia, macrophages, T cells, and B cells.\u003cbr\u003e\nFunction: Ion channel activated by extracellular ATP.\u003cbr\u003e\nLigand\/Receptor: ATP, NAD.\u003cbr\u003e\nCell Type: B cells, Macrophages, Mast cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nAntigen References: 1. Surprenant A, et al. 1996. Science. 272:735. 2. Chused TM, et al. 1996. J. Immunol. 157:1371. 3. Gargett CE, et al. 1997. Br. J. Pharmacol. 122:911. 4. Kawamura H, et al. 2006. J. Immunol. 176:2152. 5. Pelegrin P. 2011. Br. J. Pharmacol. 163:908. 6. Bartlett R, et al. 2014. Pharmacol. Rev. 66:638. 7. Alves LA, et al. 2014. Biochim. Biophys. Acta. 1838:2578.\u003cbr\u003e\nGene ID: 18439\u003cbr\u003e\nUniProt: View information about P2X7R on UniProt.org\u003cbr\u003e\nClone: 1F11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: P2X7 receptor (P2X7R), P2RX7\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860858228905,"sku":"148702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-148702","provider":"Iright","version":"1.0","type":"link"}