{"product_id":"biolegend-152319","title":"Biolegend, 152319, Alexa Fluor® 647 anti-mouse CD3ε Antibody, 25μg","description":"\u003cp\u003eCD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α\/β or γ\/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide\/MHC antigen complex.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Syrian Hamster\u003cbr\u003e\nImmunogen: Mouse T cell receptor.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: IHC-F - Quality testedFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10 µg\/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Clone 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Miescher GC, et al. 1989. Immunol. Lett. 23:113.\u003cbr\u003e\nProduct Citations: Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed\u003cbr\u003e\nRRID: AB_2750181 (BioLegend Cat. No. 152319) AB_2750182 (BioLegend Cat. No. 152320)\u003cbr\u003e\nStructure: Ig superfamily, forms CD3\/TCR complex with CD3δ, γ and ζ subunits and TCR (α\/β and γ\/δ), 20 kD.\u003cbr\u003e\nDistribution: Thymocytes (differentiation dependent), mature T cells and NK-T cells.\u003cbr\u003e\nFunction: TCR signal transduction, T cell activation and antigen recognition.\u003cbr\u003e\nInteraction: T-cell receptor (TCR).\u003cbr\u003e\nLigand\/Receptor: Peptide antigen\/MHC-complex.\u003cbr\u003e\nCell Type: NKT cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nMolecular Family: CD Molecules, TCRs\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leucocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell. 76:263.\u003cbr\u003e\nGene ID: 12501\u003cbr\u003e\nUniProt: View information about CD3epsilon on UniProt.org\u003cbr\u003e\nClone: 500A2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD3ε, T3, CD3\u003cbr\u003e\nIsotype: Syrian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862427193513,"sku":"152319","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-152319","provider":"Iright","version":"1.0","type":"link"}