{"product_id":"biolegend-152405","title":"Biolegend, 152405, PerCP\/Cyanine5.5 anti-mouse CD19 Antibody, 25μg","description":"\u003cp\u003eCD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Transfected k562 cells expressing the extracellular domain of murine CD19.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: blocking1,2 and immunoprecipitation1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Krop I, et al. 1996. Eur. J. Immunol. 26:238. (FC, Blocking, IP) Abraham P, et al. 2014. Clin. Exp. Immunol. 175:181. (Blocking)\u003cbr\u003e\nProduct Citations: Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed Pandey SP, et al. 2022. Cell Host Microbe. 30:1003. PubMed Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Li Y, et al. 2023. Adv Mater. 35:e2208923. PubMed Mansouri S, et al. 2022. J Immunol. 209:2114. PubMed Blomberg OS 2023. Cancer Cell. 41(1):106-123.e10. PubMed Li DD, et al. 2022. Cell Host Microbe. 30:530. PubMed Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed Tu X, et al. 2022. Nat Commun. 13:6977. PubMed Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed Dane EL, et al. 2022. Nat Mater. 21:710. PubMed Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed Dai G, et al. 2021. mSphere. 6: . PubMed Ronsard L, et al. 2021. Front Immunol. 12:730471. PubMed Takahashi F, et al. 2022. iScience. 25:104278. PubMed McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed Monzon-Casanova E, et al. 2021. Eur J Immunol. 51:2266. PubMed Wu L, et al. 2020. Cancer Immunol Res. 710:8. PubMed Lee A, et al. 2022. Nat Commun. 13:549. PubMed Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed\u003cbr\u003e\nRRID: AB_2629814 (BioLegend Cat. No. 152405) AB_2629815 (BioLegend Cat. No. 152406)\u003cbr\u003e\nStructure: Ig superfamily, associates with CD21 and CD81, 95 kD.\u003cbr\u003e\nDistribution: Pro-B cells to mature B cells (during development), follicular dendritic cells.\u003cbr\u003e\nFunction: Modulates B cell activation and differentiation.\u003cbr\u003e\nLigand\/Receptor: CD21, CD81, Leu-13.\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437.\u003cbr\u003e\nGene ID: 12478\u003cbr\u003e\nUniProt: View information about CD19 on UniProt.org\u003cbr\u003e\nClone: 1D3\/CD19\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B4, CVID3\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860852166825,"sku":"152405","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-152405","provider":"Iright","version":"1.0","type":"link"}