{"product_id":"biolegend-152408","title":"Biolegend, 152408, PE anti-mouse CD19 Antibody, 100μg","description":"\u003cp\u003eCD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Transfected k562 cells expressing the extracellular domain of murine CD19.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: blocking1,2 and immunoprecipitation1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Krop I, et al. 1996. Eur. J. Immunol. 26:238. (FC, Blocking, IP) Abraham P, et al. 2014. Clin. Exp. Immunol. 175:181. (Blocking)\u003cbr\u003e\nProduct Citations: Lin J, et al. 2022. Adv Sci (Weinh). 9:e2202633. PubMed Jia L, et al. 2022. Gut Microbes. 14:2117503. PubMed Cheng K, et al. 2023. Small. 19:e2300125. PubMed Sang H, et al. 2023. Adv Clin Exp Med. 32:551. PubMed Pioli KT, et al. 2023. iScience. 26:106223. PubMed Tan X, et al. 2023. Adv Sci (Weinh). 10:e2206768. PubMed Simpson AP, et al. 2022. Cell Rep. 40:111099. PubMed Shen Z, et al. 2022. EBioMedicine. 85:104285. PubMed Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed Kurosawa M, et al. 2021. Int J Mol Sci. 22:. PubMed Westerterp M et al. 2017. Cell metabolism. 25(6):1294-1304 . PubMed Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed Amor C, et al. 2020. Nature. 583:127. PubMed Zhou Y, et al. 2022. Nat Commun. 13:2906. PubMed Luo W, et al. 2022. Front Immunol. 13:816761. PubMed Cha SE, et al. 2021. Oncoimmunology. 10:1899469. PubMed Halpern KB, et al. 2018. Nat Biotechnol. 36:962. PubMed Kobayashi M, et al. 2020. Bio Protoc. 10:e3601. PubMed Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed Tartey S, et al. 2021. Cancer Research. 81(9):2358-2372. PubMed Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed La Rose AM, et al. 2021. Mol Metab. 53:101265. PubMed Zeng Z, et al. 2022. Oncogene. :. PubMed Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed\u003cbr\u003e\nRRID: AB_2629816 (BioLegend Cat. No. 152407) AB_2629817 (BioLegend Cat. No. 152408)\u003cbr\u003e\nStructure: Ig superfamily, associates with CD21 and CD81, 95 kD.\u003cbr\u003e\nDistribution: Pro-B cells to mature B cells (during development), follicular dendritic cells.\u003cbr\u003e\nFunction: Modulates B cell activation and differentiation.\u003cbr\u003e\nLigand\/Receptor: CD21, CD81, Leu-13.\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437.\u003cbr\u003e\nGene ID: 12478\u003cbr\u003e\nUniProt: View information about CD19 on UniProt.org\u003cbr\u003e\nClone: 1D3\/CD19\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B4, CVID3\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860851970217,"sku":"152408","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-152408","provider":"Iright","version":"1.0","type":"link"}