{"product_id":"biolegend-154319","title":"Biolegend, 154319, Brilliant Violet 421™ anti-mouse Perforin Antibody, 50μg","description":"\u003cp\u003ePerforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse perforin transfected cells.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Clone S16009A reacts with perforin from both C57BL\/6 and BALB\/c strains.\u003cbr\u003e\nRRID: AB_3083137 (BioLegend Cat. No. 154319)\u003cbr\u003e\nStructure: 70 kD Protein that oligomerizes for pore formation.\u003cbr\u003e\nDistribution: CTL, NK (Cytoplasmic Granules)\u003cbr\u003e\nFunction: Target cell lysis\u003cbr\u003e\nCell Type: NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: Podack ER, et al. 1988. Immunol. Rev. 103:203. Konjar S, et al. 2010. Immunology. 131:257. Trapani JA, et al. 2002. Nat. Rev. Immunol. 2:735. Osinska I, et al. 2014. Cent. Eur. J. Immnol. 39:109.\u003cbr\u003e\nGene ID: 18646\u003cbr\u003e\nUniProt: View information about Perforin on UniProt.org\u003cbr\u003e\nClone: S16009A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Prf1, Pfp, Pfn, Pfn-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866222874793,"sku":"154319","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-154319","provider":"Iright","version":"1.0","type":"link"}