{"product_id":"biolegend-154405","title":"Biolegend, 154405, PE anti-mouse Perforin Antibody, 25μg","description":"\u003cp\u003ePerforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse perforin transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone S16009B reacts with perforin from both C57BL\/6 and BALB\/c strains.\u003cbr\u003e\nProduct Citations: Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed Cuff AO, et al. 2020. Frontiers in Immunology. 1.930555556. PubMed Dumas AA, et al. 2020. EMBO J. 39:e103790. PubMed Zhang B, et al. 2021. Nature. 599:471. PubMed Li X, et al. 2021. Front Cell Dev Biol. 9:647713. PubMed\u003cbr\u003e\nRRID: AB_2721640 (BioLegend Cat. No. 154405) AB_2721641 (BioLegend Cat. No. 154406)\u003cbr\u003e\nStructure: 70 kD Protein that oligomerizes for pore formation.\u003cbr\u003e\nDistribution: CTL, NK (Cytoplasmic Granules)\u003cbr\u003e\nFunction: Target cell lysis\u003cbr\u003e\nCell Type: T cells, NK cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: Podack ER, et al, 1988, Immunol Rev, 103:203 Konjar S, et al, 2010. Immunology, 131:257 Trapani JA, et al, 2002, Nat Rev Immunol, 2:735 Osinska I, et al. 2014, Cent Eur J Immnol, 39:109\u003cbr\u003e\nGene ID: 18646\u003cbr\u003e\nUniProt: View information about Perforin on UniProt.org\u003cbr\u003e\nClone: S16009B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Prf1, Pfp, Pfn, Pfn-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866222907561,"sku":"154405","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-154405","provider":"Iright","version":"1.0","type":"link"}