{"product_id":"biolegend-155508","title":"Biolegend, 155508, APC anti-mouse CD170 (Siglec-F) Antibody, 100μg","description":"\u003cp\u003eCD170, also known as Siglec-F and Siglec-5, is a member of the sialic acid-binding immunoglobulin-like lectin (siglec) family of type I single pass transmembrane proteins.  Siglec-F has four extracellular Ig-like domains and two cytoplasmic ITIM motifs and preferentially binds [alpha]-2,3-linked sialic acid. Siglec-F is expressed on eosinophils, alveolar macrophages and intestinal microfold (M) cells and induces apoptosis of lung eosinophils during allergic asthma.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nProduct Citations: Larson-Casey JL, et al. 2023. JCI Insight. 8:. PubMed Gonçalves R, et al. 2023. iScience. 26:105972. PubMed He J, et al. 2022. Front Immunol. 12:760138. PubMed Larson-Casey JL, et al. 2021. FASEB J. 35:e21675. PubMed Pionnier NP, et al. 2019. Nat Commun. 10:1429. PubMed Antunes KH, et al. 2022. Front Immunol. 13:867022. PubMed Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed Daneshmandi S, et al. 2021. Elife. 10:. PubMed Larson-Casey JL, et al. 2021. J Biol Chem. :100810. PubMed Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed Rochford I, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321:L686. PubMed Larson–Casey JL, et al. 2019. J Clin Invest. 129:4962. PubMed Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed\u003cbr\u003e\nRRID: AB_2750236 (BioLegend Cat. No. 155507) AB_2750237 (BioLegend Cat. No. 155508)\u003cbr\u003e\nStructure: Member of the Sialic acid-binding Ig-like lectin family, type I, single pass, transmembrane protein, 4 extracellular Ig-like domains, 2 ITIM motifs in the cytoplasmic domain\u003cbr\u003e\nDistribution: Eosinophils, alveolar macrophages, intestinal microfold (M) cells\u003cbr\u003e\nFunction: Induces apoptosis in eosinophils\u003cbr\u003e\nLigand\/Receptor: [alpha]-2,3-linked sialic acid\u003cbr\u003e\nCell Type: Eosinophils, Macrophages\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Siglec Molecules\u003cbr\u003e\nAntigen References: Gicheva N, et al. 2016. Biochem. Biophys. Res. Commun. 479:1. Kiwamoto T, et al. 2015. J. Allergy Clin. Immunol. 135:1329. Suzukawa M, et al. 2013. J. Immunol. 190:5939. Patnode ML, et al. 2013. J. Biol. Chem. 288:26533.\u003cbr\u003e\nGene ID: 233186\u003cbr\u003e\nUniProt: View information about CD170 on UniProt.org\u003cbr\u003e\nClone: S17007L\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Siglec-5\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866228904105,"sku":"155508","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-155508","provider":"Iright","version":"1.0","type":"link"}