{"product_id":"biolegend-155509","title":"Biolegend, 155509, Brilliant Violet 421™ anti-mouse CD170 (Siglec-F) Antibody, 50μg","description":"\u003cp\u003eCD170, also known as Siglec-F and Siglec-5, is a member of the sialic acid-binding immunoglobulin-like lectin (siglec) family of type I single pass transmembrane proteins.  Siglec-F has four extracellular Ig-like domains and two cytoplasmic ITIM motifs and preferentially binds [alpha]-2,3-linked sialic acid. Siglec-F is expressed on eosinophils, alveolar macrophages and intestinal microfold (M) cells and induces apoptosis of lung eosinophils during allergic asthma.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nProduct Citations: Petenkova A, et al. 2023. Nat Commun. 14:2761. PubMed Hardman CS, et al. 2022. Nat Commun. 13:7535. PubMed Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed Stump CT, et al. 2021. Open Biol. 11:210245. PubMed Sung PS, et al. 2022. JCI Insight. :. PubMed Yu L, et al. 2022. Front Immunol. 13:937331. PubMed\u003cbr\u003e\nRRID: AB_2810421 (BioLegend Cat. No. 155509)\u003cbr\u003e\nStructure: Member of the Sialic acid-binding Ig-like lectin family, type I, single pass, transmembrane protein, 4 extracellular Ig-like domains, 2 ITIM motifs in the cytoplasmic domain\u003cbr\u003e\nDistribution: Eosinophils, alveolar macrophages, intestinal microfold (M) cells\u003cbr\u003e\nFunction: Induces apoptosis in eosinophils\u003cbr\u003e\nLigand\/Receptor: [alpha]-2,3-linked sialic acid\u003cbr\u003e\nCell Type: Eosinophils, Macrophages\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Siglec Molecules\u003cbr\u003e\nAntigen References: Gicheva N, et al. 2016. Biochem. Biophys. Res. Commun. 479:1. Kiwamoto T, et al. 2015. J. Allergy Clin. Immunol. 135:1329. Suzukawa M, et al. 2013. J. Immunol. 190:5939. Patnode ML, et al. 2013. J. Biol. Chem. 288:26533.\u003cbr\u003e\nGene ID: 233186\u003cbr\u003e\nUniProt: View information about CD170 on UniProt.org\u003cbr\u003e\nClone: S17007L\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Siglec-5\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866229690537,"sku":"155509","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-155509","provider":"Iright","version":"1.0","type":"link"}