{"product_id":"biolegend-156551","title":"Biolegend, 156551, StarBright UltraViolet 740 anti-mouse NK-1.1 Antibody, VO31","description":"\u003cp\u003eThis StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For ordering assistance or to request a sample, contact your local field representative .   This product is also available for direct purchase within a StarBright Basic Panel , which combines a minimal set of pre-selected compatible reagents. NK-1.1 surface antigen, also known as CD161b\/CD161c and Ly-55, is encoded by the NKR-P1B\/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL\/6, FVB\/N, and NZB, but not AKR, BALB\/c, CBA\/J, C3H, DBA\/1, DBA\/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo . NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.\u003cbr\u003e\nVO31\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Mouse NK1.1-transfectants\u003cbr\u003e\nFormulation: Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 740 under optimal conditions.\u003cbr\u003e\nConcentration: 0.6 mg\/mL (the concentration relates to the dye component of the conjugate)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * StarBright UltraViolet 740 has a maximum excitation of 343 nm and a maximum emission of 737 nm. StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.\u003cbr\u003e\nExcitation Laser: Ultraviolet Laser (355 nm)\u003cbr\u003e\nApplication Notes: Clone S17016D cross-blocks anti-mouse NK1.1 clone PK136, and can stain for NK1.1 post-formaldehyde and methanol-based fixation based on in-house testing.\u003cbr\u003e\nStructure: NKR-P1 gene family\u003cbr\u003e\nDistribution: NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB\/N, NZB)\u003cbr\u003e\nFunction: NK cell activation, IFN-γ production, cytotoxic granule release\u003cbr\u003e\nCell Type: NK cells, NKT cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nAntigen References: Lanier LL. 1997. Immunity 6:371. Yokoyama WM, Seaman WE. 1993. Annu. Rev. Immunol. 11:613. Koo GC, et al. 1986. J. Immunol. 137:3742. Giorda R, Trucco M. 1991. J. Immunol. 147:1701.\u003cbr\u003e\nGene ID: 17059\u003cbr\u003e\nUniProt: View information about NK-1.1 on UniProt.org\u003cbr\u003e\nClone: S17016D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: NKR-P1C, NKR-P1B, Ly-55, CD161b, CD161c\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: What is a “StarBright panel”?\u003cbr\u003e\nA: A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.\u003cbr\u003e\nQ: Will my StarBright panel be provided as a cocktail?\u003cbr\u003e\nA: StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.\u003cbr\u003e\nQ: Are StarBright GMP\/ASR products available?\u003cbr\u003e\nA: StarBright ASR and GMP products are coming soon.\u003cbr\u003e\nQ: Are StarBright custom products available?\u003cbr\u003e\nA: Yes, please contact our Custom Solutions Team.\u003cbr\u003e\nQ: Will StarBright isotype controls be offered?\u003cbr\u003e\nA: We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.\u003cbr\u003e\nQ: What amount of a StarBright conjugate should I use?\u003cbr\u003e\nA: Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.\u003cbr\u003e\nQ: Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?\u003cbr\u003e\nA: For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866236276905,"sku":"156551","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-156551","provider":"Iright","version":"1.0","type":"link"}