{"product_id":"biolegend-160004","title":"Biolegend, 160004, PE anti-mouse CD287 (TLR7) Antibody, 100μg","description":"\u003cp\u003eToll-like receptor (TLR) 7, also known as CD287 is a type 1 transmembrane protein, member of the TLR receptor family. TLR7 is expressed intracellularly in plasmacytoid dendritic cells, myeloid cells, and B lymphocytes and plays an important role in the innate immune response by binding single-stranded RNA (ssRNA). TLR7 signals through MyD88, which leads to the activation and secretion of IFN-α\/β and other inflammatory cytokines.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Ba\/F3 cells expressing flag-6xHis-conjugated mouse TLR7\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kanno A, et al. 2013. Int Immunol. 25:413\u003cbr\u003e\nProduct Citations: Mulder EJ, et al. 2024. Front Immunol. 1340384:15. PubMed Guenther C, et al. 2024. Front Immunol. 1434030:15. PubMed Raiten J, et al. 2024. Front Pharmacol. 1471563:15. PubMed\u003cbr\u003e\nRRID: AB_2860749 (BioLegend Cat. No. 160003) AB_2876562 (BioLegend Cat. No. 160004)\u003cbr\u003e\nStructure: Type 1 transmembrane protein, member of the Toll-like receptor (TLR) family\u003cbr\u003e\nDistribution: Myeloid and plasmacytoid dendritic cells, and B lymphocytes\u003cbr\u003e\nFunction: Pattern recognition receptor that detects single-stranded RNA, induces proinflammatory cytokine production\u003cbr\u003e\nLigand\/Receptor: Single-stranded RNA\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Innate Immune Signaling\u003cbr\u003e\nAntigen References: Majer O, et al. 2019. Nature. 575:371. Akilesh HM, et al. 2019. Science 363(6423) Hemmi H, et al. 2002. Nat. Immunol. 3:196.\u003cbr\u003e\nGene ID: 170743\u003cbr\u003e\nUniProt: View information about CD287 on UniProt.org\u003cbr\u003e\nClone: A94B10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: TLR7, Toll-like receptor 7, CD287\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862526152873,"sku":"160004","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-160004","provider":"Iright","version":"1.0","type":"link"}