{"product_id":"biolegend-161205","title":"Biolegend, 161205, PE\/Fire™ 810 anti-mouse CD62L Antibody, 25μg","description":"\u003cp\u003eCD62L is a 74-95 kD glycoprotein also known as L-selectin. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte \"rolling\" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse CD62L recombinant protein (39-332)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Fire™ 810 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE\/Fire™ 810 has a maximum excitation of 488\/561 nm and a maximum emission of 810 nm. Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE\/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nProduct Citations: Bloodworth N, et al. 2024. J Clin Invest. :134. PubMed\u003cbr\u003e\nRRID: AB_2910338 (BioLegend Cat. No. 161205)\u003cbr\u003e\nStructure: CD62L is a type I transmembrane glycoprotein with 74-95 kD.\u003cbr\u003e\nDistribution: CD62L is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells.\u003cbr\u003e\nFunction: CD62L plays roles in lymphocyte homing to lymphoid organs and inflammatory leukocyte trafficking.\u003cbr\u003e\nLigand\/Receptor: CD34, GlyCAM-1, MAdCAM-1\u003cbr\u003e\nCell Type: B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Rosen S. 2004. Annu. Rev. Immunol. 22:129 2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.\u003cbr\u003e\nGene ID: 20343\u003cbr\u003e\nUniProt: View information about CD62L on UniProt.org\u003cbr\u003e\nClone: W18021D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: L-selectin\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866329075881,"sku":"161205","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-161205","provider":"Iright","version":"1.0","type":"link"}