{"product_id":"biolegend-163403","title":"Biolegend, 163403, TruStain FcX™ PLUS (Human IgG4 anti-mouse CD16\/32) Recombinant Antibody, 50μg","description":"\u003cp\u003eCD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16\/CD32 are expressed on B cells, monocytes\/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ PLUS (Human IgG4 anti-mouse CD16\/32) is specific to the common epitope of CD16\/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors and is more effective than TruStain FcX™.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Recombinant\u003cbr\u003e\nHost Species: Human\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain FcX™ PLUS (Human IgG4 anti-mouse CD16\/32) for 5 - 10 minutes, on ice, at 0.25 µg per 106 cells in a volume of 100 µL, prior to immunostaining. It is not necessary to wash the cells between the blocking and immunostaining steps.\u003cbr\u003e\nProduct Citations: Xu H, et al. 2023. J Biomed Res. 340:37. PubMed\u003cbr\u003e\nRRID: AB_2922498 (BioLegend Cat. No. 163403) AB_2922498 (BioLegend Cat. No. 163404)\u003cbr\u003e\nStructure: Ig superfamily, 40-60 kD\u003cbr\u003e\nDistribution: B cells, monocyte\/macrophages, NK cells, neutrophils, mast cells, dendritic cells\u003cbr\u003e\nLigand\/Receptor: IgG\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nGene ID: 1413014131\u003cbr\u003e\nClone: QA19A30\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD16, CD32\u003cbr\u003e\nIsotype: Human IgG4, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866188304553,"sku":"163403","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-163403","provider":"Iright","version":"1.0","type":"link"}