{"product_id":"biolegend-200501","title":"Biolegend, 200501, Purified anti-rat CD252 (OX-40 Ligand) Antibody, 50μg","description":"\u003cp\u003eOX-40 Ligand (OX-40L) is a 22 kD member of the TNF superfamily (TNFSF4), also known as CD252 or CD134L. It is expressed on dendritic cells, peritoneal macrophages, and activated B cells. The interaction of OX-40L with OX-40 (CD134) is important for B cell antibody production and T cell activation and proliferation. The ATM-2 antibody is able to block the costimulatory function of OX-40L.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: ACI-S1 rat T-cell lymphoma cell line\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: blocking the binding of OX-40 to OX-40L and the inhibition of T cell costimulatory activity of OX40L1. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 200506) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 200504) or biotinylated anti-mouse IgG (Cat. No. 405303) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 200508).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Satake, Y., et al. 2000. Biochem. Biophys. Res. Commun. 270:1041.\u003cbr\u003e\nRRID: AB_2571881 (BioLegend Cat. No. 200501)\u003cbr\u003e\nStructure: TNF superfamily, 22 kD\u003cbr\u003e\nDistribution: Dendritic cells, activated B cells\u003cbr\u003e\nFunction: T cell dependent humoral responses\u003cbr\u003e\nLigand\/Receptor: OX40 (CD134)\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Satake, Y., et al. 2000. Biochem. Biophys. Res. Commun. 270:1041. 2. Akiba, H., et al. 1998. Biochem. Biophys. Res. Commun. 251:131.3. Akiba, H., et al. 2000. J. Exp. Med. 191:375.\u003cbr\u003e\nGene ID: 22164\u003cbr\u003e\nUniProt: View information about CD252 on UniProt.org\u003cbr\u003e\nClone: ATM-2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: OX-40 Ligand, CD134L, TNFSF4\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862543945897,"sku":"200501","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-200501","provider":"Iright","version":"1.0","type":"link"}