{"product_id":"biolegend-201520","title":"Biolegend, 201520, PerCP\/Cyanine5.5 anti-rat CD4 Antibody, 100μg","description":"\u003cp\u003eCD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Rat thymocyte membrane glycoproteins\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: The W3\/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC) Shioji K, et al. 2001. Circulation Res. 89:540. (IHC) Mannie MD, et al. 1993. J. Immunol. 151:7293. Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed\u003cbr\u003e\nProduct Citations: Zhang Y, et al. 2024. Biology (Basel). :13. PubMed Giri T, et al. 2022. Heliyon. 8:e09407. PubMed\u003cbr\u003e\nRRID: AB_2814097 (BioLegend Cat. No. 201519) AB_2814098 (BioLegend Cat. No. 201520)\u003cbr\u003e\nStructure: Ig superfamily, 55 kD\u003cbr\u003e\nDistribution: Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)\u003cbr\u003e\nFunction: T cell co-receptor, T cell activation\u003cbr\u003e\nLigand\/Receptor: MHC class II molecule\u003cbr\u003e\nCell Type: Macrophages, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609. 2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649.\u003cbr\u003e\nGene ID: 24932\u003cbr\u003e\nUniProt: View information about CD4 on UniProt.org\u003cbr\u003e\nClone: W3\/25\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T4, L3T4, W3\/25\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864085483689,"sku":"201520","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-201520","provider":"Iright","version":"1.0","type":"link"}