{"product_id":"biolegend-260098","title":"Biolegend, 260098, GMP PE anti-human CD3 Antibody, 100tests","description":"\u003cp\u003eCD3ε is a 20 kD chain of the CD3\/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α\/β or γ\/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.\u003cbr\u003e\n100tests\u003cbr\u003e\nReactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w\/v) BSA (origin USA) and a stabilizer.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 25 µg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kan EA, et al. 1983. J. Immunol. 131:536. Wood GS, et al. 1985. Am. J. Pathol. 120:371. Van Dongen JJM, et al. 1988. Blood 71:603. (WB) Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC) Carbone A, et al. 1999. Blood 93:2319. (IHC) Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF) Rutjens E, et al. 2007. J. Immunol. 178:1702. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC) Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)\u003cbr\u003e\nDisclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products\u003cbr\u003e\nStructure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α\/β or γ\/δ) forms CD3\/TCR complex, 20 kD\u003cbr\u003e\nDistribution: Mature T and NK T cells, during thymocyte differentiation\u003cbr\u003e\nFunction: Antigen recognition, signal transduction, T cell activation\u003cbr\u003e\nLigand\/Receptor: Peptide antigen bound to MHC\u003cbr\u003e\nCell Type: NKT cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, TCRs\u003cbr\u003e\nAntigen References: Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. Lanier L, et al. 1986. J. Immunol. 137:2501.\u003cbr\u003e\nGene ID: 916\u003cbr\u003e\nUniProt: View information about CD3 on UniProt.org\u003cbr\u003e\nClone: SK7\u003cbr\u003e\nOther Names: T3, CD3ε\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866527617193,"sku":"260098","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-260098","provider":"Iright","version":"1.0","type":"link"}