{"product_id":"biolegend-260354","title":"Biolegend, 260354, GMP PE\/Cyanine7 anti-human CD27 Antibody, 100tests","description":"\u003cp\u003eCD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70, and plays a role in costimulation of T cell activation and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK\/JNK activation.\u003cbr\u003e\n100tests\u003cbr\u003e\nReactivity: Human\u003cbr\u003e\nReported Reactivity: Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human T cells from a T-ALL patient.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w\/v) BSA (origin USA) and a stabilizer.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 100 µg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections1, immunofluorescent staining2, and ELISA3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ma S, et al. 2011. J. Virol. 85:165. (IHC) Manzo A, et al. 2008. Arthritis Rheum. 11:3377. (IF) Kato K, et al. 2007. Exp. Hematol. 35:434. (ELISA)\u003cbr\u003e\nDisclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products\u003cbr\u003e\nStructure: TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD\u003cbr\u003e\nDistribution: Medullary thymocytes, T and B cell subsets, NK cells\u003cbr\u003e\nInteraction: CD27 binds to CD70\u003cbr\u003e\nLigand\/Receptor: CD70\u003cbr\u003e\nCell Type: B cells, NK cells, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. 2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. 3. Hintzen R, et al. 1994. Immunol. Today 15:307. 4. Agematsu K, et al. 1995. J. Immunol. 154:3627.\u003cbr\u003e\nGene ID: 939\u003cbr\u003e\nUniProt: View information about CD27 on UniProt.org\u003cbr\u003e\nClone: M-T271\u003cbr\u003e\nWorkshop: V 5T CD27.03\u003cbr\u003e\nOther Names: S152, T14, TNFRSF7\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866525814953,"sku":"260354","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-260354","provider":"Iright","version":"1.0","type":"link"}