{"product_id":"biolegend-301639","title":"Biolegend, 301639, Purified anti-human CD11c (Maxpar® Ready) Antibody, 100μg","description":"\u003cp\u003eCD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin α X and CR4. CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes\/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Chimpanzee, Squirrel Monkey\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 1.0 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested CyTOF® - Verified\u003cbr\u003e\nRecommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.\u003cbr\u003e\nApplication Notes: Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent12. While analyzing blood, it is best to use heparin as the anti-coagulant and not EDTA. Since the ability of clone 3.9 to bind to its target is divalent cation dependent, the usage of EDTA as an anti-coagulant may be detrimental to staining due to its chelating properties. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4, and functional assays5,6. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301616). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin \u0026lt;0.01 EU\/µg).\u003cbr\u003e\nAdditional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York. Vainer B, et al. 2000. Am. J. Surg. Pathol. 24:1115. (IHC) Ottonello L, et al. 1999. Blood 93:3505. Metelitsa LS, et al. 2002. Blood 99:4166. Sadhu C, et al. 2007. J. Leukoc. Biol. doi:10.1189\/jlb.1106680. PubMed Ihanus E, et al. 2007. Blood 109:802-810. Gurer C, et al. 2008. Blood 112:1231. PubMed Asai A, et al. 2009. J. Lipid Res. 50:95. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. (FC)\u003cbr\u003e\nProduct Citations: Van Tilbeurgh M, et al. 2022. Cell Rep Med. 3:100751. PubMed Stensland ZC, et al. 2022. iScience. 25:103626. PubMed\u003cbr\u003e\nRRID: AB_2562812 (BioLegend Cat. No. 301639)\u003cbr\u003e\nStructure: Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD\u003cbr\u003e\nDistribution: Myeloid, dendritic cells, NK cells, B cells and T cell subsets\u003cbr\u003e\nFunction: Adhesion, CTL killing\u003cbr\u003e\nLigand\/Receptor: CD54, fibrinogen, iC3b, ICAM-1, ICAM-4\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Neutrophils, NK cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Petty H. 1996. Immunol. Today 17:209. 2. Springer T. 1994. Cell 76:301. 3. Ihanus E, et al. 2007. Blood 109:802-810.\u003cbr\u003e\nGene ID: 3687\u003cbr\u003e\nUniProt: View information about CD11c on UniProt.org\u003cbr\u003e\nClone: 3.9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: III NL707\u003cbr\u003e\nOther Names: Integrin αX subunit, CR4, p150, ITGAX\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?\u003cbr\u003e\nA: We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.\u003c\/p\u003e\n\n\u003cp\u003ehttp:\/\/techsupport.fluidigm.com\/\u003cbr\u003e\nQ: Can I use Maxpar® Ready format clones for flow cytometry staining?\u003cbr\u003e\nA: We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.\u003cbr\u003e\nQ: I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.\u003cbr\u003e\nA: We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http:\/\/techsupport.fluidigm.com\/\u003cbr\u003e\nQ: Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?\u003cbr\u003e\nA: The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862333935785,"sku":"301639","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-301639","provider":"Iright","version":"1.0","type":"link"}