{"product_id":"biolegend-302001","title":"Biolegend, 302001, Purified anti-human CD16 Antibody, 25μg","description":"\u003cp\u003eCD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human PMN cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested CyTOF® - Verified Block, IHC-F, IP, Stim - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Edberg J, et al. 1997. J. Immunol. 159:3849. (IP) Hoshino S, et al. 1991. Blood 78:3232. (Stim) Tamm A, et al. 1996. Immunol. 157:1576. (Block) Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC) Holl V, et al. 2004. J. Immunol. 173:6274. (Block) Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block) Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. (Block) PubMed Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Rout N, et al. 2010. PLoS One 5:e9787. (FC) Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC) Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed Wu Z, et al. 2013. J. Virol. 87:7717. PubMed Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG) Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim)\u003cbr\u003e\nProduct Citations: Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed Dorsch M, et al. 2020. Eur J Immunol. 50:656. PubMed Alpert A, et al. 2022. Cell Syst. 13:71. PubMed McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed Shemesh A, et al. 2022. J Exp Med. 219: . PubMed Kirolos SA, et al. 2022. J Leukoc Biol. 112:1399. PubMed Miyamoto DT, et al. 2018. Cancer Discov. 0.533333333. PubMed Weinhage T, et al. 2020. J Immunol. 205:56. PubMed Wu Z, et al. 2013. J Virol. 87:7717. PubMed Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed Korn MA, et al. 2020. J Immunol. 205:2595. PubMed Friedman D, et al. 2021. J Cell Sci. 134:. PubMed Smed-Sörensen A, et al. 2008. Blood. 111:5037. PubMed Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed Fasbender F, et al. 2017. Front Immunol. 0.88125. PubMed Holder KA, et al. 2021. Clin Transl Immunology. 10:e1348. PubMed Santos R, et al. 2017. Nat Commun. . 10.1038\/s41467-017-01760-5. PubMed Mask E, et al. 2022. Front Cell Infect Microbiol. 12:880860. PubMed Mann ER, et al. 2020. Sci Immunol. :5. PubMed Boltz A, et al. 2011. J Biol Chem. 286:21896. PubMed Winkler M, et al. 2017. PLoS One.. 10.1371\/journal.pone.0182427. PubMed Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed Li L, et al. 2019. Cell Stem Cell. 24:271. PubMed Gainers M, et al. 2007. J Immunol. 179:8509. PubMed Sibener LV et al. 2018. Cell. 174(3):672-687 . PubMed Okubo K, et al. 2021. Cell Reports. 35(7):109142. PubMed Urlaub D, et al. 2017. J Immunol. 198:1944. PubMed Syrimi E, et al. 2021. iScience. 24:103215. PubMed Jog NR, et al. 2018. Front Immunol. 9:2198. PubMed Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed Olin A, et al. 2018. Cell. 174:1277. PubMed Farhadian SF, et al. 2022. JCI Insight. 7:. PubMed Park JE, et al. 2020. Journal for ImmunoTherapy of Cancer. 8(2):e000873.. PubMed Auladell M, et al. 2019. Clin Transl Immunology. 8:e01090. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Prodjinotho UF, et al. 2017. PLoS Negl Trop Dis. 11:e0005777. PubMed Park YJ, et al. 2022. PLoS Pathog. 18:e1010468. PubMed Frobel J, et al. 2018. Clin Epigenetics. 10:67. PubMed Ehinger E, et al. 2021. Cardiovasc Res. 117:1166. PubMed Mody P, et al. 2007. Blood. 110:2974. PubMed Ardain A, et al. 2019. Nature. 570:528. PubMed Li J, et al. 2021. Haematologica. 106:3115. PubMed Shemesh A, et al. 2022. J Exp Med. 219:. PubMed Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed Combes AJ, et al. 2021. Nature. 591:124. PubMed Steffen U, et al. 2020. Nat Commun. 0.541666667. PubMed Boutboul D, et al. 2018. J Clin Invest. 128:3071. PubMed Pohl K, et al. 2018. PLoS One. 13:e0209026. PubMed Doni A, et al. 2021. Nat Commun. 12:3739. PubMed De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed Ravindran A, et al. 2018. Front Immunol. 9:2193. PubMed Farrington LA, et al. 2020. PLoS Pathog. 16:e1008997. PubMed Edri-Brami M, et al. 2012. PLoS One. 7:e35772. PubMed Lakshmikanth T, et al. 2020. Cell Reports. 32(3):107923. PubMed Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed Sung JA, et al. 2018. Mol Ther. 26:2496. PubMed Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed Zhong Q, et al. 2018. J Immunol. 200:3913. PubMed Ravell JC, et al. 2020. J Clin Invest. 130:507. PubMed Martin E, et al. 2020. JCI Insight. :5. PubMed Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed\u003cbr\u003e\nRRID: AB_314201 (BioLegend Cat. No. 302001) AB_314202 (BioLegend Cat. No. 302002)\u003cbr\u003e\nStructure: Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)\u003cbr\u003e\nDistribution: NK cells, activated monocytes, macrophages, neutrophils\u003cbr\u003e\nFunction: Low affinity IgG Fc receptor, phagocytosis, ADCC\u003cbr\u003e\nLigand\/Receptor: Aggregated IgG, IgG-antigen complex\u003cbr\u003e\nCell Type: Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nAntigen References: 1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275. 2. Stroncek D, et al. 1991. Blood 77:1572. 3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.\u003cbr\u003e\nGene ID: 2214\u003cbr\u003e\nUniProt: View information about CD16 on UniProt.org\u003cbr\u003e\nClone: 3G8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V NK80\u003cbr\u003e\nOther Names: FcγRIII, Fc gamma receptor, Fc gamma receptor 3\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?\u003cbr\u003e\nA: Yes\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862398128297,"sku":"302001","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-302001","provider":"Iright","version":"1.0","type":"link"}