{"product_id":"biolegend-302606","title":"Biolegend, 302606, PE anti-human CD25 Antibody, 100tests","description":"\u003cp\u003eCD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes\/macrophages. CD25 is also expressed on a subset of non-stimulated CD4 + T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The CD25 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications include: immunocytochemistry3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed\u003cbr\u003e\nProduct Citations: Riner D, et al. 2016. PLoS Negl Trop Dis. 10:e0005180. PubMed Tang M, et al. 2019. Biomed Res Int. 2019:1050285. PubMed Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed Yang M, et al. 2019. Cell Physiol Biochem. 52:1178. PubMed Ando M, et al. 2021. Cancer Res Commun. 1:41. PubMed Hu Y, et al. 2023. Front Immunol. 14:1128423. PubMed Pfister F, et al. 2023. Methods Mol Biol. 2644:331. PubMed Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed Alam A, et al. 2022. Cancer Cell. 40:153. PubMed Hong Y, et al. 2022. J Leukoc Biol. 112:425. PubMed Zhang H, et al. 2023. Nat Immunol. 24:96. PubMed Xu Q, et al. 2023. Nat Immunol. 24:186. PubMed Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed Netsrithong R, et al. 2020. Stem Cell Research. 39:101487.. PubMed Freeman ZT, et al. 2020. J Clin Invest. 130:1405. PubMed Miao L, et al. 2020. Theranostics. 0.7625. PubMed Li X, et al. 2019. Mol Med Rep. 19:4195. PubMed Savage TM, et al. 2018. JCI Insight. 3:. PubMed Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed Saadati S, et al. 2020. Avicenna J Med Biotechnol. 0.591666667. PubMed Wei H, et al. 2021. Malar J. 20:89. PubMed Xun X, et al. 2021. Am J Transl Res. 13:4360. PubMed Vinogradova EV, et al. 2021. STAR Protoc. 2:100458. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Goletz C, et al. 2018. Front Immunol. 9:1614. PubMed de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed Lopatina T, et al. 2018. Sci Rep. 8:17458. PubMed Ramien C, et al. 2020. Cell Reports. 29(4):810-815. PubMed Pothlichet J, et al. 2022. Front Immunol. 13:824746. PubMed Bellini N, et al. 2022. iScience. 25:105234. PubMed Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed James E, et al. 2016. PLoS Pathog. 12: 1005375. PubMed Chen Y, et al. 2012. PLoS One. 7:e47190. PubMed Soodgupta D, et al. 2019. Cell Rep. 29:829. PubMed Meng S, et al. 2018. Mol Med Rep. 18:4247. PubMed Zhang W, et al. 2012. J Biol Chem. 287:34157. PubMed Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed Robertson N, et al. 2020. 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STAR Protoc. 2:100864. PubMed Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed Lima J, et al. 2016. Reprod Sci. 10.1177\/1933719116653680. PubMed Keefe RC, et al. 2021. Sci Rep. 11:14933. PubMed Tian M, et al. 2021. Elife. 10:. PubMed Chruewkamlow N, et al. 2021. Stem Cell Res Ther. 12:520. PubMed Imura Y, et al. 2020. JCI Insight. :5. PubMed Vinogradova EV, et al. 2020. Cell. 182(4):1009-1026.e29. PubMed Han L, et al. 2014. J Biol Chem. 289:25546. PubMed Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed Zhang B, et al. 2020. Cell Chemical Biology. 28(1):60-69.e7. PubMed Ferraro A, et al. 2014. Proc Natl Acad Sci U S A. 111:1111. PubMed Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed Bal S, et al. 2016. Nat Immunol. 10.1038\/ni.3444. PubMed Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed Ajith A, et al. 2019. FASEB J. 33:5220. PubMed Wang L, et al. 2018. Oncol Lett. 15:8635. PubMed Cerny D, et al. 2016. J Allergy Clin Immunol. 138:1709-1712. PubMed Fierle JK, et al. 2021. Cell Reports Medicine. 2(8):100362. PubMed Robertson N, et al. 2020. J Biol Chem. 295:18436. PubMed Welch JL, et al. 2020. FEBS Lett. 594:695. PubMed Wang AS, et al. 2020. Molecular Cell. 79(2):221-233.e5.. PubMed Kim N, et al. 2020. Nat Commun. 2.045138889. PubMed Hui Z, et al. 2022. Cell Death Dis. 13:607. PubMed Högelin KA, et al. 2021. iScience. 24:103078. PubMed Choe JH, et al. 2021. Sci Transl Med. 13:. PubMed\u003cbr\u003e\nRRID: AB_314275 (BioLegend Cat. No. 302605) AB_314276 (BioLegend Cat. No. 302606)\u003cbr\u003e\nStructure: Type I transmembrane glycoprotein, 55 kD\u003cbr\u003e\nDistribution: Activated T cells and B cells, monocytes\/macrophages, Treg\u003cbr\u003e\nFunction: Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex\u003cbr\u003e\nLigand\/Receptor: IL-2\u003cbr\u003e\nCell Type: B cells, Macrophages, Monocytes, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann T. 1991. J. Biol. Chem. 266:2681.\u003cbr\u003e\nGene ID: 3559\u003cbr\u003e\nUniProt: View information about CD25 on UniProt.org\u003cbr\u003e\nClone: BC96\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V T-072\u003cbr\u003e\nOther Names: Low affinity IL-2R, IL-2R α chain, Tac, p55\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862903509161,"sku":"302606","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-302606","provider":"Iright","version":"1.0","type":"link"}