{"product_id":"biolegend-302617","title":"Biolegend, 302617, Alexa Fluor® 647 anti-human CD25 Antibody, 25tests","description":"\u003cp\u003eCD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes\/macrophages. CD25 is also expressed on a subset of non-stimulated CD4 + T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm \/ 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications include: immunocytochemistry3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed\u003cbr\u003e\nProduct Citations: Zhang H, et al. 2023. Nat Immunol. 24:96. PubMed Blomberg OS 2023. Cancer Cell. 41(1):106-123.e10. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Tiittanen M, et al. 2013. PLoS One. 7:78420. PubMed Kilpelainen A, et al. 2022. Front Immunol. 13:815041. PubMed Singh KS, et al. 2021. Nature. 589:597. PubMed Keck S, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 12(2):507-545. PubMed\u003cbr\u003e\nRRID: AB_493046 (BioLegend Cat. No. 302617) AB_493045 (BioLegend Cat. No. 302618)\u003cbr\u003e\nStructure: Type I transmembrane glycoprotein, 55 kD\u003cbr\u003e\nDistribution: Activated T cells and B cells, monocytes\/macrophages, Treg\u003cbr\u003e\nFunction: Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex\u003cbr\u003e\nLigand\/Receptor: IL-2\u003cbr\u003e\nCell Type: B cells, Macrophages, Monocytes, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann T. 1991. J. Biol. Chem. 266:2681.\u003cbr\u003e\nGene ID: 3559\u003cbr\u003e\nUniProt: View information about CD25 on UniProt.org\u003cbr\u003e\nClone: BC96\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V T-072\u003cbr\u003e\nOther Names: Low affinity IL-2R, IL-2R α chain, Tac, p55\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862902984873,"sku":"302617","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-302617","provider":"Iright","version":"1.0","type":"link"}