{"product_id":"biolegend-302904","title":"Biolegend, 302904, Biotin anti-human CD28 Antibody, 100μg","description":"\u003cp\u003eCD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The Ultra-LEAF™ Purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Nunes J, et al. 1993. Biochem. J. 293:835. Calea-Lauri J, et al. 1999. J. Immunol. 163:62. Tazi A, et al. 1999. J. Immunol. 163:3511. (IHC) Marti F, et al. 2001. J. Immunol. 166:197. (Costim) Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim) Rivollier A, et al. 2004. Blood 104:4029. (Costim) Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Costim) Sheng W, et al. 2007.Elsevier 580:6819. PubMed Mitsuhashi M. 2007. Clin Chem.53:148. PubMed Ye Z, et al. 2008. Infect. Immun. 76:2541. PubMed Magatti M, et al. 2008. Stem Cells 26:182. (FA) PubMed Yoshino N, et al. 2008. Exp. Anim. (Tokyo) 49:97. (FC) Berg M, et al. 2008. J Leukoc Biol. 83:853. (IP) PubMed Rout N, et al. 2010. PLoS One 5:e9787. (FC) Leonard JA, et al. 2011. J. Virol. 85:6867. PubMed Nomura T, et al. 2012. J. Virol. 86:6481. PubMed\u003cbr\u003e\nProduct Citations: Zhang DKY, et al. 2020. Nature Protocols. 15(3):773-798.. PubMed Lui VG, et al. 2023. J Clin Immunol. . PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Eller MA, et al. 2016. J Virol. 90: 4005-4016. PubMed Serwas NK, et al. 2019. Nat Commun. 2.573611111. PubMed Zheng Y, et al. 2021. Nat Commun. 12:3580. PubMed\u003cbr\u003e\nRRID: AB_314305 (BioLegend Cat. No. 302903) AB_314306 (BioLegend Cat. No. 302904)\u003cbr\u003e\nStructure: Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD\u003cbr\u003e\nDistribution: Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells\u003cbr\u003e\nFunction: T cell costimulation\u003cbr\u003e\nLigand\/Receptor: CD80, CD86\u003cbr\u003e\nCell Type: B cells, NK cells, Plasma cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. June CH, et al. 1994. Immunol. Today 15:321. 3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191.\u003cbr\u003e\nGene ID: 940\u003cbr\u003e\nUniProt: View information about CD28 on UniProt.org\u003cbr\u003e\nClone: CD28.2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V-CD28.05\u003cbr\u003e\nOther Names: T44, Tp44\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862977695913,"sku":"302904","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-302904","provider":"Iright","version":"1.0","type":"link"}