{"product_id":"biolegend-302926","title":"Biolegend, 302926, PE\/Cyanine7 anti-human CD28 Antibody, 100tests","description":"\u003cp\u003eCD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: The Ultra-LEAF™ Purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Nunes J, et al. 1993. Biochem. J. 293:835. Calea-Lauri J, et al. 1999. J. Immunol. 163:62. Tazi A, et al. 1999. J. Immunol. 163:3511. (IHC) Marti F, et al. 2001. J. Immunol. 166:197. (Costim) Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim) Rivollier A, et al. 2004. Blood 104:4029. (Costim) Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Costim) Sheng W, et al. 2007.Elsevier 580:6819. PubMed Mitsuhashi M. 2007. Clin Chem.53:148. PubMed Ye Z, et al. 2008. Infect. Immun. 76:2541. PubMed Magatti M, et al. 2008. Stem Cells 26:182. (FA) PubMed Yoshino N, et al. 2008. Exp. Anim. (Tokyo) 49:97. (FC) Berg M, et al. 2008. J Leukoc Biol. 83:853. (IP) PubMed Rout N, et al. 2010. PLoS One 5:e9787. (FC) Leonard JA, et al. 2011. J. Virol. 85:6867. PubMed Nomura T, et al. 2012. J. Virol. 86:6481. PubMed\u003cbr\u003e\nProduct Citations: Liu L, et al. 2016. Nat Biotechnol. 10.1038\/nbt.3461. PubMed Li M, et al. 2021. J Clin Invest. 131:. PubMed Sudmeier LJ, et al. 2022. Cell Rep Med. 3:100620. PubMed Arasanz H, et al. 2022. Cancers (Basel). 14: . PubMed Fujita T, et al. 2014. J Immunol. 193:5576. PubMed Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed Teteloshvili N, et al. 2018. Front Immunol. 1.347222222. PubMed Zuazo M, et al. 2019. EMBO Mol Med. 11(7). PubMed Ramien C, et al. 2020. Cell Reports. 29(4):810-815. PubMed Kerstein A, et al. 2016. J Autoimmun. S0896-8411(16)30186-X. PubMed Edwards CJ, et al. 2021. Br J Cancer. . PubMed Leitner J, et al. 2015. J Immunol. 195: 477 - 487. PubMed Martins M, et al. 2015. J Virol. 89: 10802 - 10820. PubMed Xue J, et al. 2013. PLoS One. 8:72295. PubMed Aalam F, et al. 2020. PLoS Pathog. 16:e1008968. PubMed\u003cbr\u003e\nRRID: AB_10644004 (BioLegend Cat. No. 302925) AB_10644005 (BioLegend Cat. No. 302926)\u003cbr\u003e\nStructure: Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD\u003cbr\u003e\nDistribution: Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells\u003cbr\u003e\nFunction: T cell costimulation\u003cbr\u003e\nLigand\/Receptor: CD80, CD86\u003cbr\u003e\nCell Type: B cells, NK cells, Plasma cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. June CH, et al. 1994. Immunol. Today 15:321. 3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191.\u003cbr\u003e\nGene ID: 940\u003cbr\u003e\nUniProt: View information about CD28 on UniProt.org\u003cbr\u003e\nClone: CD28.2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V-CD28.05\u003cbr\u003e\nOther Names: T44, Tp44\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862980710569,"sku":"302926","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-302926","provider":"Iright","version":"1.0","type":"link"}