{"product_id":"biolegend-303016","title":"Biolegend, 303016, Alexa Fluor® 488 anti-human CD29 Antibody, 100tests","description":"\u003cp\u003eCD29 is a 130 kD single chain type I glycoprotein also known as integrin β 1 , VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins, which include CD29, bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cow, Cynomolgus, Dog, Horse, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen tissue sections3,5, and activation of integrin ß14,7,8. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303010). Clone TS2\/16 recognizes epitope A2.10\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208. Hemler ME, et al. 1984. J. Immunol. 132:3011. (IHC, IP) Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ) Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) Murga M, et al. 2005. Blood 105:1992. (FC) PubMed Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ) Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ) Wesseling J, et al. 1995. J. Cell. Biol. 129:255. (Dog Reactivity) Rubio G, et al. 2002. Cancer Immunol. Immunother. 51:130. Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed Paebst F, et al. 2014. Cytometry A. 85(8):678-87. (Horse reactivity)\u003cbr\u003e\nProduct Citations: Porat-Shliom N, et al. 2013. PLoS One. 25:81897. PubMed Mendt M, et al. 2018. JCI Insight. 9:1523. PubMed Bayik D, et al. 2022. Cancer Res. 82:4274. PubMed Huber M, et al. 2023. Nat Commun. 14:2143. PubMed Shao X, et al. 2023. Cell Discov. 9:44. PubMed Ge W, et al. 2022. Int J Mol Sci. 24: . PubMed Buffone A, et al. 2018. J Cell Sci. 131:. PubMed Esteves C, et al. 2017. Stem Cell Res Ther. 10.1186\/s13287-017-0525-2. PubMed Bitton A, et al. 2021. Cytometry A. . PubMed Morad G, et al. 2020. International Journal of Molecular Sciences. 21(11):3851. PubMed Paebst F, et al. 2016. Cytometry A. 85:678-87. PubMed Esteves C and Donadeu F. 2017. Cytometry A. 10.1002\/cyto.a.23243. PubMed Ayhan S, et al. 2021. Journal of Cell Science. 134(6):. PubMed Liu Z, et al. 2021. Cells. 10:. PubMed Tanigawa K, et al. 2019. J Cell Physiol. 234:17280. PubMed Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed Ding S, et al. 2018. Sci Rep. 8:10808. PubMed Rogers MA, et al. 2022. Front Cardiovasc Med. 9:778919. PubMed Le AH, et al. 2021. Journal of Cell Biology. 220(9):. PubMed\u003cbr\u003e\nRRID: AB_493026 (BioLegend Cat. No. 303015) AB_493025 (BioLegend Cat. No. 303016)\u003cbr\u003e\nStructure: Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α1-α6), also associates with CD51 (αV), and α7- α9, 130 kD\u003cbr\u003e\nDistribution: Lymphocytes, monocytes, granulocytes (low), platelets, mast cells, fibroblasts, endothelial cells\u003cbr\u003e\nFunction: Cell-cell and cell-matrix interactions\u003cbr\u003e\nLigand\/Receptor: VCAM-1, MAdCAM-1, ECM\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Endothelial cells, Fibroblasts, Granulocytes, Lymphocytes, Mast cells, Mesenchymal Stem Cells, Monocytes, Platelets, Tregs\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Hemler M. 1990. Annu. Rev. Immunol. 8:365. 2. Hynes R. 1992. Cell 69:11.\u003cbr\u003e\nGene ID: 3688\u003cbr\u003e\nUniProt: View information about CD29 on UniProt.org\u003cbr\u003e\nClone: TS2\/16\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V A-S202\u003cbr\u003e\nOther Names: Integrin β1 chain, VLA-β chain, gpIIa, ITGB1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864021979305,"sku":"303016","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-303016","provider":"Iright","version":"1.0","type":"link"}