{"product_id":"biolegend-303102","title":"Biolegend, 303102, Purified anti-human CD31 Antibody, 100μg","description":"\u003cp\u003eCD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested ELISA Capture, ICC - Verified IHC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. For ELISA Capture applications, a concentration of 1.0 µg\/mL is recommended. To obtain a linear standard curve, serial dilutions of CD31 recombinant protein ranging from 10 to 0.156 ng\/mL is recommended for each ELISA plate. For immunocytochemistry, a concentration range of 2.5 - 10 μg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 \u0026amp; 303144).The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF) Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block) Nagano M, et al. 2007. Blood 110:151. (FC) PubMed MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Critchley WR, et al. 2023. J Cell Sci. 136:. PubMed Yuge S, et al. 2022. Nat Commun. 13:2594. PubMed Setua S, et al. 2022. Sci Rep. 12:22191. PubMed Xie T, et al. 2021. Sci Adv. 7: . PubMed Robert J et al. 2017. eLife. 6 pii: e29595. PubMed Bloodworth NC, et al. 2018. Circ Res. 123:e51. PubMed Constantinescu-Bercu A, et al. 2020. eLife. 9:00. PubMed Fitzgerald W, et al. 2018. Am J Reprod Immunol. 80:e12860. PubMed Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed Vukusic K, et al. 2019. Stem Cells Dev. 28:1078. PubMed Olin A, et al. 2018. Cell. 174:1277. PubMed Yoshitomi Y, et al. 2017. J Cell Sci. 130:916. PubMed Nagano M, et al. 2007. Blood . 110:151. PubMed Boulanger MD, et al. 2022. PLoS One. 17:e0269316. PubMed Henrick BM, et al. 2021. Cell. . PubMed Lundqvist A, et al. 2016. PLoS One. 11: 0161629. PubMed Li X, et al. 2022. Cancer Cell Int. 22:157. PubMed Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed Lee SH, et al. 2022. Nat Commun. 13:5461. PubMed Lakshmikanth T, et al. 2020. Cell Reports. 32(3):107923. PubMed Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed Fujiwara K, et al. 2020. Cancers (Basel). 12:. PubMed Gunasekaran M, et al. 2018. J Immunol. 200:2535. PubMed Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed Robert J, et al. 2017. Mol Neurodegener. 12:60. PubMed Crawford LB, et al. 2021. J Virol. 95:. PubMed Li H, et al. 2020. Haematologica. 105:1206. PubMed Martin E, et al. 2020. JCI Insight. :5. PubMed\u003cbr\u003e\nRRID: AB_314327 (BioLegend Cat. No. 303101) AB_314328 (BioLegend Cat. No. 303102)\u003cbr\u003e\nStructure: Ig superfamily, type I transmembrane glycoprotein, 130-140 kD\u003cbr\u003e\nDistribution: Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset\u003cbr\u003e\nFunction: Cell adhesion, signal transduction\u003cbr\u003e\nLigand\/Receptor: CD38\u003cbr\u003e\nCell Type: Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: DeLisser H, et al. 1994. Immunol. Today 15:490. Newman P, 1997. J. Clin. Invest. 99:3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229.\u003cbr\u003e\nGene ID: 5175\u003cbr\u003e\nUniProt: View information about CD31 on UniProt.org\u003cbr\u003e\nClone: WM59\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V P025\u003cbr\u003e\nOther Names: PECAM-1, EndoCAM\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864096428201,"sku":"303102","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-303102","provider":"Iright","version":"1.0","type":"link"}