{"product_id":"biolegend-303119","title":"Biolegend, 303119, APC\/Cyanine7 anti-human CD31 Antibody, 25tests","description":"\u003cp\u003eCD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 \u0026amp; 303144).The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF) Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block) Nagano M, et al. 2007. Blood 110:151. (FC) PubMed MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Mendt M, et al. 2018. JCI Insight. 9:1523. PubMed Wu J, et al. 2022. Nat Commun. 13:676. PubMed Wu X, et al. 2022. Front Immunol. 13:811551. PubMed Zarobkiewicz M, et al. 2022. J Clin Med. 11: . PubMed Flaskamp L, et al. 2022. Cells. 11: . PubMed Basil MC, et al. 2022. Nature. 604:120. PubMed Lin GL, et al. 2018. Acta Neuropathol Commun. 9:617. PubMed Webster K, et al. 2013. Hum Reprod. 28:651. PubMed Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed Philippeos C, et al. 2018. J Invest Dermatol. 138:811. PubMed Panciera T, et al. 2020. Nat Mater. 1.345138889. PubMed Kathiriya JJ, et al. 2022. Nat Cell Biol. 24:10. PubMed Piekarska K, et al. 2022. Nat Commun. 13:856. PubMed Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed Spearman AD, et al. 2021. Front Pediatr. 9:679572. PubMed Wu X, et al. 2018. J Immunol Methods. 455:71. PubMed Xi H, et al. 2020. Cell Stem Cell. 27(1):158-176. PubMed Phansalkar R, et al. 2021. Elife. 10:. PubMed Xu J et al. 2019. Elife. 8 pii: e48191. PubMed Camunas–Soler J, et al. 2020. Cell Metabolism. 31(5):1017-1031. PubMed Haynes BA, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2168. PubMed\u003cbr\u003e\nRRID: AB_10643590 (BioLegend Cat. No. 303119) AB_10640734 (BioLegend Cat. No. 303120)\u003cbr\u003e\nStructure: Ig superfamily, type I transmembrane glycoprotein, 130-140 kD\u003cbr\u003e\nDistribution: Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset\u003cbr\u003e\nFunction: Cell adhesion, signal transduction\u003cbr\u003e\nLigand\/Receptor: CD38\u003cbr\u003e\nCell Type: Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: DeLisser H, et al. 1994. Immunol. Today 15:490. Newman P, 1997. J. Clin. Invest. 99:3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229.\u003cbr\u003e\nGene ID: 5175\u003cbr\u003e\nUniProt: View information about CD31 on UniProt.org\u003cbr\u003e\nClone: WM59\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V P025\u003cbr\u003e\nOther Names: PECAM-1, EndoCAM\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864095707305,"sku":"303119","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-303119","provider":"Iright","version":"1.0","type":"link"}