{"product_id":"biolegend-303408","title":"Biolegend, 303408, APC anti-human CD33 Antibody, 100tests","description":"\u003cp\u003eCD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate\/lectin binding activity.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Chimpanzee\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human myeloid leukaemia cells.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 303437 \u0026amp; 303438).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB) Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)\u003cbr\u003e\nProduct Citations: Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed Harder L, et al. 2013. J Exp Med. 210:2289. PubMed Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed Frame JM, et al. 2020. Developmental Cell. 55(2):133-149.e6. PubMed Grenga I, et al. 2016. Clin Transl Immunology. 0.265972222. PubMed Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed Maat H, et al. 2021. iScience. 24(5):102435. PubMed Abd Hamid M, et al. 2020. Cancer Immunology Research. 8(2):203-216. PubMed Arvindam US, et al. 2021. Leukemia. 35:1586. PubMed Audigé A, et al. 2017. BMC Immunology. 10.1186\/s12865-017-0209-9. PubMed Burel JG, et al. 2019. Elife. 8. PubMed\u003cbr\u003e\nRRID: AB_314351 (BioLegend Cat. No. 303407) AB_314352 (BioLegend Cat. No. 303408)\u003cbr\u003e\nStructure: Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD\u003cbr\u003e\nDistribution: Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells\u003cbr\u003e\nFunction: Adhesion and lectin activity\u003cbr\u003e\nLigand\/Receptor: Sugar chains containing sialic acid\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules, Siglec Molecules\u003cbr\u003e\nAntigen References: 1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. 2. Freeman S, et al. 1995. Blood 85:2005.\u003cbr\u003e\nGene ID: 945\u003cbr\u003e\nUniProt: View information about CD33 on UniProt.org\u003cbr\u003e\nClone: WM53\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: IV M-505\u003cbr\u003e\nOther Names: Siglec-3, gp67, p67\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864092397737,"sku":"303408","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-303408","provider":"Iright","version":"1.0","type":"link"}