{"product_id":"biolegend-303653","title":"Biolegend, 303653, FITC Recombinant Protein L, 50tests","description":"\u003cp\u003eProtein L was initially isolated from bacteria Peptostreptococcus magnus cell wall, and now is prepared as recombinant protein. Protein L has been found to have a high affinity for Ig κ light chains. This unique ability of protein L to bind to the κ light chains of antibodies makes it valuable in detection and purification of antibodies and antibody fragments that contain kappa light chains but not the Fc region, such as antigen-binding VL domains and Fv fragments prepared from monoclonal antibodies. It is also used to detect chimeric antigen receptor (CAR) expressing cells.\u003cbr\u003e\n50tests\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: For multi-color flow cytometry analysis, cells should be stained with Protein L first, and washed; costaining reagent(s) need to be applied as the second step.\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Cell Biology, Immuno-Oncology, Immunology\u003cbr\u003e\nAntigen References: Björck L. 1988. J Immunol. 140:1194-7. Akerström B and Björck L. 1989. J Biol Chem. 264:19740-6.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about Protein L on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866494685353,"sku":"303653","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-303653","provider":"Iright","version":"1.0","type":"link"}