{"product_id":"biolegend-304319","title":"Biolegend, 304319, Purified anti-human CD49d (Maxpar® Ready) Antibody, 100μg","description":"\u003cp\u003eCD49d is a 150 kD α integrin chain known as α 4 integrin or VLA-4 α chain. It forms a heterodimer with either integrin β1 (α 4 β 1 , VLA-4) or β7 (α 4 β 7 ). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. α 4 β 7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation. CD49d is a marker to isolate pure populations of Treg cells due to its absence on Foxp3 + cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cat, Cow, Chimpanzee, Common Marmoset, Dog, Horse, Sheep, Squirrel Monkey\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 1.0 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested CyTOF® - Verified\u003cbr\u003e\nRecommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, and in vitro T cell costimulation2,3. The Ultra-LEAF™ Purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304339 and 304340).\u003cbr\u003e\nAdditional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim) Vogel TU, et al. 2002. J. Immunol. 169:4511. (Costim) Kleinewietfeld M, et al. 2009. Blood 113:827. (FC) PubMed Palacious F, et al. 2010. Blood 115:4488. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. Mattapallil MJ, et al. 2011. J. Immunol. 187:1977. PubMed\u003cbr\u003e\nProduct Citations: Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed\u003cbr\u003e\nRRID: AB_2563755 (BioLegend Cat. No. 304319)\u003cbr\u003e\nStructure: Integrin, type I transmembrane glycoprotein, 150 kD.\u003cbr\u003e\nDistribution: T cells, B cells, NK , dendritic cells, thymocytes, monocytes, eosinophils, mast cells.\u003cbr\u003e\nFunction: Lymphocyte migration, T cell activation, stem cell differentiation.\u003cbr\u003e\nLigand\/Receptor: Fibronectin, VCAM-1, MAdCAM-1\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Elices M, Ed.1995. Springer Semin. Immunopathol. 16(4). 2. Lobb RR and Helmer ME. et al. 1994. J. Clin. Invest. 94:1722.\u003cbr\u003e\nGene ID: 3676\u003cbr\u003e\nUniProt: View information about CD49d on UniProt.org\u003cbr\u003e\nClone: 9F10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V S215\u003cbr\u003e\nOther Names: VLA-4 α chain, α4 integrin, Integrin α4 chain, ITGA4\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?\u003cbr\u003e\nA: We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.\u003c\/p\u003e\n\n\u003cp\u003ehttp:\/\/techsupport.fluidigm.com\/\u003cbr\u003e\nQ: Can I use Maxpar® Ready format clones for flow cytometry staining?\u003cbr\u003e\nA: We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.\u003cbr\u003e\nQ: I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.\u003cbr\u003e\nA: We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http:\/\/techsupport.fluidigm.com\/\u003cbr\u003e\nQ: Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?\u003cbr\u003e\nA: The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862513111209,"sku":"304319","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-304319","provider":"Iright","version":"1.0","type":"link"}