{"product_id":"biolegend-304822","title":"Biolegend, 304822, PE\/Cyanine7 anti-human CD62L Antibody, 100tests","description":"\u003cp\u003eCD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Chimpanzee, Cow\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Concentrated supernatant from PMA-activated human peripheral blood leukocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: Western blotting2,3,9 and in vitro blocking of lymphocytes binding to high endothelial venules (HEV)2. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 304853-304858).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kishimoto TK, et al. 1990. Proc. Natl. Acad. Sci. USA 87:2244. (WB, Block) Jutila M, et al. 2002. J. Immunol. 169:1768. (WB) Tamassia N, et al. 2008. J. Immunol. 181:6563. (FC) PubMed Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Koenig JM, et al. 1996. Pediatr. Res. 39:616. (WB) Shi C, et al. 2011. J. Immunol. 187:5293. (FC) PubMed Burges M, et al. 2013. Clin Cancer Res. 19:5675. PubMed Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed\u003cbr\u003e\nProduct Citations: Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed Zhou Y, et al. 2021. Methods Mol Biol. 2388:35. PubMed Graciliano NG, et al. 2023. Front Immunol. 13:1031248. PubMed Ye L, et al. 2022. Cell Metab. 34:595. PubMed Das RK, et al. 2019. Cancer Discov. 9:492. PubMed Kim C, et al. 2018. Cell Rep. 25:2148. PubMed Konadu K, et al. 2015. J Infect Dis. 211:1712. PubMed Shi Z, et al. 2021. Ann Clin Transl Neurol. 8:43. PubMed Giamarellos-Bourboulis EJ, et al. 2020. Cell. 183(2):315-323.e9. PubMed Toews K, et al. 2020. Mol Carcinog. 724:59. PubMed Moorlag SJCFM, et al. 2020. Cell Rep. 33:108387. PubMed Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed Theobald SJ, et al. 2022. EMBO Mol Med. 14:e15888. PubMed Hu N, et al. 2014. PLoS One. 9:99671. PubMed Singh N, et al. 2016. Sci Transl Med. 8: 320ra3. PubMed Veerapathran A, et al. 2011. Blood. 118:5671. PubMed Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed Liao HQ, et al. 2021. Am J Reprod Immunol. 86:e13406. PubMed Crompton J, et al. 2015. Cancer Res. 75:296. PubMed Zou F, et al. 2019. Nat Commun. 10:4109. PubMed\u003cbr\u003e\nRRID: AB_830800 (BioLegend Cat. No. 304821) AB_830801 (BioLegend Cat. No. 304822)\u003cbr\u003e\nStructure: Selectin, single chain glycoprotein, 74-95 kD\u003cbr\u003e\nDistribution: Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes\u003cbr\u003e\nFunction: Leukocyte homing, leukocyte tethering, rolling\u003cbr\u003e\nLigand\/Receptor: CD34, GlyCAM, MAdCAM-1\u003cbr\u003e\nCell Type: B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244. Kishimoto T, et al. 1991. Blood 78:805.\u003cbr\u003e\nGene ID: 6402\u003cbr\u003e\nUniProt: View information about CD62L on UniProt.org\u003cbr\u003e\nClone: DREG-56\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V S056\u003cbr\u003e\nOther Names: L-selectin, LECAM-1, LAM-1, Leu-8, TQ-1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863014887593,"sku":"304822","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-304822","provider":"Iright","version":"1.0","type":"link"}