{"product_id":"biolegend-304904","title":"Biolegend, 304904, FITC anti-human CD62P (P-Selectin) Antibody, 100tests","description":"\u003cp\u003eCD62P is a 140 kD type I transmembrane glycoprotein also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Chimpanzee\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4 and in vitro blocking of adhesion of platelets1. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304947 \u0026amp; 304948).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Skinner M, et al. 1991. J. Biol. Chem. 266:5371. (Block) Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Yen YT, et al. 2006. J. Virol. 80:2684. Sato Y, et al. 2005. Blood 106:428. (IHC)\u003cbr\u003e\nProduct Citations: Ma X, et al. 2023. Research (Wash D C). 6:0124. PubMed Takayama N, et al. 2010. J Exp Med. 207:2817. PubMed Slingsby MHL, et al. 2021. Haematologica. Online ahead of print. PubMed Long C, et al. 2020. Toxins (Basel). 12:00. PubMed Hijmans JG, et al. 2019. J Am Heart Assoc. 8:e011134. PubMed Bhat A, et al. 2020. Hepatol Commun. 4:50. PubMed Jiang Y, et al. 2021. Cell Death Differ. 28:3009. PubMed Duan X, et al. 2021. Sci Rep. 11:13170. PubMed Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed Reitsma SE, et al. 2021. Am J Physiol Cell Physiol. 320:C365. PubMed Ansari M, et al. 2017. Cell Signal. 10.1016\/j.cellsig.2017.04.014. PubMed Yen Y, et al. 2006. J Virol. 80:2684. PubMed Gillespie S, et al. 2018. Br J Pharmacol. 175:3333. PubMed Dregalla RC, et al. 2021. J Orthop Res. 39:2744. PubMed Costa B, et al. 2019. Blood Adv. 3:1092. PubMed\u003cbr\u003e\nRRID: AB_314475 (BioLegend Cat. No. 304903) AB_314476 (BioLegend Cat. No. 304904)\u003cbr\u003e\nStructure: Selectin, type I glycoprotein, 140 kD\u003cbr\u003e\nDistribution: Activated platelets, megakaryocytes, endothelial cells\u003cbr\u003e\nFunction: Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions\u003cbr\u003e\nLigand\/Receptor: CD162 (PSGL-1), CD24 and sialylated Lewis X\u003cbr\u003e\nCell Type: Endothelial cells, Megakaryocytes, Neutrophils, Platelets\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. McEver R, et al. 1995. J. Biol. Chem. 270:11025. 2. Varki A. 1994. P. Natl. Acad. Sci. USA 91:7390.\u003cbr\u003e\nGene ID: 6403\u003cbr\u003e\nUniProt: View information about CD62P on UniProt.org\u003cbr\u003e\nClone: AK4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: VI P-44\u003cbr\u003e\nOther Names: GMP-140, PADGEM\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862537523369,"sku":"304904","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-304904","provider":"Iright","version":"1.0","type":"link"}