{"product_id":"biolegend-305203","title":"Biolegend, 305203, Biotin anti-human CD80 Antibody, 25μg","description":"\u003cp\u003eCD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305245 \u0026amp; 305246).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC) Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB) Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed Wen T, et al. 2014. J Immunol. 192:5481. PubMed\u003cbr\u003e\nProduct Citations: Lee J, et al. 2015. J Exp Med. 212:385. PubMed\u003cbr\u003e\nRRID: AB_314500 (BioLegend Cat. No. 305204)\u003cbr\u003e\nStructure: Ig superfamily, type I transmembrane glycoprotein, 60 kD\u003cbr\u003e\nDistribution: Activated B cells and T cells, macrophages, and dendritic cells\u003cbr\u003e\nFunction: T cell costimulation\u003cbr\u003e\nLigand\/Receptor: CD28, CD152 (CTLA-4)\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Freeman G, et al. 1991. J. Exp. Med. 174:625. 2. Linsley P, et al. 1996. Immunity 4:535. 3. Linsley P, et al. 1991. J. Exp. Med. 174:561.\u003cbr\u003e\nGene ID: 941\u003cbr\u003e\nUniProt: View information about CD80 on UniProt.org\u003cbr\u003e\nClone: 2D10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: VI CD80.1\u003cbr\u003e\nOther Names: B7-1, B7, BB1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862305951913,"sku":"305203","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-305203","provider":"Iright","version":"1.0","type":"link"}