{"product_id":"biolegend-305602","title":"Biolegend, 305602, Purified anti-human CD95 (Fas) Antibody, 100μg","description":"\u003cp\u003eCD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils, and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis and has been shown to play a role in the maintenance of peripheral tolerance.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Pigtailed Macaque, Sooty Mangabey\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: CD95 transfected L cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested ICC - Verified IHC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis3 (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 305655 and 305656). Note: EOS9.1 antibody can induce apoptosis without cross-linking.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds.1995. Leucocyte Typing V. Oxford University Press. New York. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York. Cifone M, et al. 1994. J. Exp. Med. 180:1547. (Apop) Zietz C, et al. 2001. Am. J. Pathol. 159:963. (IHC) Sergi C, et al. 2000. Am. J. Pathol. 156:1589. (IHC) Xie S, et al. 2010. J. Immunol. 184:2289. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. Rout N, et al. 2010. PLoS One 5:e9787. (FC) Dixit N, et al. 2012. J. Immunol. 189:5954. PubMed\u003cbr\u003e\nProduct Citations: Dixit N, et al. 2012. J Immunol. 189:5954. PubMed Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed Ortiz AM, et al. 2018. Nat Med. 24:1313. PubMed Sharma S, et al. 2019. Nat Commun. 2.572916667. PubMed Nugent JL, et al. 2021. iScience. 24:103421. PubMed Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed Ortiz AM, et al. 2022. Sci Rep. 12:7491. PubMed Zhang Y, et al. 2020. Front Immunol. 11:1012. PubMed Syrimi E, et al. 2021. iScience. 24:103215. PubMed O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed Sun Y, et al. 2020. J Immunol. 205:3358. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed Sciuto MR, et al. 2018. Mol Cell Proteomics. 1.397916667. PubMed De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed\u003cbr\u003e\nRRID: AB_314540 (BioLegend Cat. No. 305602)\u003cbr\u003e\nStructure: TNFR superfamily, type I transmembrane protein, 45 kD\u003cbr\u003e\nDistribution: T cells and B cells, monocytes, neutrophils, fibroblasts, expression level upregulated on activated lymphocytes\u003cbr\u003e\nFunction: Mediates apoptosis\u003cbr\u003e\nLigand\/Receptor: Fas ligand (CD178)\u003cbr\u003e\nCell Type: B cells, Fibroblasts, Lymphocytes, Monocytes, Neutrophils, T cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Krammer P, et al. 1994. Immunol. Rev. 142:175. 2. Nagata S, et al. 1995. Science 267:1449.\u003cbr\u003e\nGene ID: 355\u003cbr\u003e\nUniProt: View information about CD95 on UniProt.org\u003cbr\u003e\nClone: DX2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: VI C-64\u003cbr\u003e\nOther Names: Fas, APO-1, TNFRSF6\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863048310953,"sku":"305602","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-305602","provider":"Iright","version":"1.0","type":"link"}