{"product_id":"biolegend-306301","title":"Biolegend, 306301, Purified anti-human CD150 (SLAM) Antibody, 25μg","description":"\u003cp\u003eCD150 is a 70-95 kD type I transmembrane glycoprotein also known as SLAM or IPO-3. It is a member of the Ig superfamily. It is expressed on a subset of T cells, B cells, dendritic cells, and endothelial cells. The expression of CD150 is upregulated upon activation. CD150 binds to itself as the ligand to be involved in B cell costimulation, proliferation, immunoglobulin production, and signal transduction.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Activated human PBMC\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IHC-F, Costim, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunoprecipitation4, and costimulation1,5 of IFN-gamma production and T cell proliferation. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt;0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306317 and 306318).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Garcia V, et al. 2001. J. Immunol. 167:5719. (Costim) Vincent S, et al. 2002. J. Virol. 76:6121. Cocks B, et al. 1995. Nature 376:260. Sayos J, et al. 2001. Blood 97:3867. (IP) Aversa G, et al. 1997. J. Immunol. 158:4036. (Costim) Spencer M, et al. 2010. Am. J. Physiol Endocrinol Metab. 299:1016. PubMed\u003cbr\u003e\nProduct Citations: Baldanzi G, et al. 2011. J Immunol. 187:5941. PubMed Spencer M, et al. 2010. Am J Physiol Endocrinol Metab. 299:E1016. PubMed\u003cbr\u003e\nRRID: AB_314590 (BioLegend Cat. No. 306302)\u003cbr\u003e\nStructure: Ig superfamily, type I transmembrane glycoprotein, 70-95 kD\u003cbr\u003e\nDistribution: T subset (upregulated after activation), B cells, dendritic cells, endothelial cells\u003cbr\u003e\nFunction: B cell costimulation, proliferation and Ig production\u003cbr\u003e\nLigand\/Receptor: CD150 (self ligand)\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Cocks B, et al. 1995. Nature 376:260. 2. Pinchouk V, et al. 1988. AntiCancer Res. 8:1377. 3. Polacino P, et al. 1996. J. Med. Primatol. 25:201. 4. Punnonen J, et al. 1997. J. Exp. Med. 185:993.\u003cbr\u003e\nGene ID: 6504\u003cbr\u003e\nUniProt: View information about CD150 on UniProt.org\u003cbr\u003e\nClone: A12 (7D4)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: SLAM, Signaling Lymphocyte Activation Molecule, IPO-3\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862517797033,"sku":"306301","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-306301","provider":"Iright","version":"1.0","type":"link"}