{"product_id":"biolegend-307006","title":"Biolegend, 307006, PE anti-human DcR1 (TRAIL-R3, CD263) Antibody, 100tests","description":"\u003cp\u003eDcR1 is a 65 kD GPI-anchored member of the TNFR superfamily (TNFRSF10C) also known as TRAIL-R3, TRID, CD263, and LIT. Although not expressed by most tumors, DcR1 is expressed at low levels on normal tissues, acting as a TRAIL decoy receptor, thus inhibiting apoptosis. DcR1 is termed a decoy receptor, as it does not have a functional death domain to induce apoptosis following TRAIL ligation.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Extracellular domain of DcR1-human IgG1 Fc fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: The DJR3 antibody is useful for immunofluorescent staining and flow cytometric analysis of DcR1\/TRAIL-R3 receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 307006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 307004) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Uno K, et al. 2003. Blood 101:3658. Sato K, et al. 2005. J. Immunol. 174:4025.\u003cbr\u003e\nProduct Citations: Greenlee JD, et al. 2021. eLife. 0.416666666666667. PubMed Chen QZ, et al. 2020. Front Immunol. 1.202777778. PubMed Staniek J, et al. 2019. Front Immunol. 10:951. PubMed\u003cbr\u003e\nRRID: AB_2287506 (BioLegend Cat. No. 307005) AB_2205089 (BioLegend Cat. No. 307006)\u003cbr\u003e\nStructure: TNFR superfamily, GPI-anchored, truncated death domain, 65 kD\u003cbr\u003e\nDistribution: Most normal tissues, not tumors\u003cbr\u003e\nFunction: TRAIL decoy, inhibits apoptosis\u003cbr\u003e\nLigand\/Receptor: TRAIL\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Schneider P, et al. 1997. J. Biol. Chem. 272:25417. 2. Pan G, et al. 1997. Science 277:815.\u003cbr\u003e\nGene ID: 8794\u003cbr\u003e\nUniProt: View information about CD263 on UniProt.org\u003cbr\u003e\nClone: DJR3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: TRAIL-R3, TRID, LIT, CD263, TNFRSF10C\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863011971241,"sku":"307006","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-307006","provider":"Iright","version":"1.0","type":"link"}