{"product_id":"biolegend-310701","title":"Biolegend, 310701, Purified anti-human CD193 (CCR3) Antibody, 25μg","description":"\u003cp\u003eCD193, also known as CC-chemokine receptor 3 (CCR3), CC CKR3, MIP1-alpha receptor like-2, and eotaxin receptor, is a member of the G protein-coupled seven transmembrane receptors family. It binds to the CC chemokines eotaxin, eotaxin-2, and eotaxin-3 with high affinity. CCR3 has also been reported to bind RANTES, MCP-3, and MCP-4 with low affinity. CCR3 receptor is expressed on human eosinophils, basophils, mast cells, mononuclear phagocytes, platelets, CD34 + hematopoietic progenitor cells, Th2-like lymphocytes, and keratinocytes. CCR3 is thought to play a role in allergic diseases such as bronchial asthma and allergic rhinitis. CCR3 is a co-receptor for HIV-1 and HIV-2, and the binding of eotaxin with CCR3 has been shown to inhibit HIV infection in some cell types.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. For immunohistochemistry on paraffin-embedded tissue, a concentration range of 5.0 - 10.0 µg\/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: The 5E8 antibody is useful for immunofluorescent staining and flow cytometric analysis of CCR3 expression. It has been observed that the 5E8 antibody clone can interact with PE\/Cyanine7 antibody conjugates during multi-color staining, potentially leading to unwanted staining. This interaction can be resolved by sequentially staining with the 5E8 antibody first and then followed by the PE\/Cyanine7 conjugate of interest.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Beauvillian C, et al. 2011. Blood 117:1196. PubMed\u003cbr\u003e\nProduct Citations: O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed Olin A, et al. 2018. Cell. 174:1277. PubMed Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed Lakshmikanth T, et al. 2020. Cell Reports. 32(3):107923. PubMed Beauvillain C, et al. 2011. Blood. 117:1196. PubMed Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed\u003cbr\u003e\nRRID: AB_345394 (BioLegend Cat. No. 310702)\u003cbr\u003e\nStructure: G-protein coupled seven transmembrane domain receptor, 356 amino acids\u003cbr\u003e\nDistribution: Eosinophils, basophils, mast, mononuclear phagocytes, platelets, CD34+ hematopoietic progenitor, Th2, keratinocytes\u003cbr\u003e\nFunction: Co-receptor for HIV-1 and HIV-2, allergy\u003cbr\u003e\nReceptors: Eotaxin, eotaxin-2, eotaxin-3\u003cbr\u003e\nCell Type: Eosinophils, Erythrocytes, Hematopoietic stem and progenitors, Macrophages, Mast cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Gerard W, et al. 1996. J. Exp. Med. 183:2437. 2. Uguccioni C, et al. 1997. J. Clin. Invest. 100:1137. 3. Sallusto F, et al. 1997. Science. 277:2005. 4. Loetscher P, et al. 2001. J. Biol. Chem. 276:2986.\u003cbr\u003e\nRegulation: Upregulated by high affinity Fc IgE receptor ligation (mast cells), RANTES (keratinocytes), IFNg (monocytes, neutrophils), HIV tat protein (basophils), IL-3, IL-5 and GM-CSF (CD34+ progenitor cells), IL-2 and IL-4(T cells). Downregulated by IL-\u003cbr\u003e\nGene ID: 1232\u003cbr\u003e\nUniProt: View information about CD193 on UniProt.org\u003cbr\u003e\nClone: 5E8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CC CKR3, MIP1-alpha receptor like-2, eotaxin receptor, CD193, CCR3\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864147906729,"sku":"310701","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-310701","provider":"Iright","version":"1.0","type":"link"}