{"product_id":"biolegend-314511","title":"Biolegend, 314511, PerCP\/Cyanine5.5 anti-human IgM Antibody, 25tests","description":"\u003cp\u003eIgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human Ig cocktail\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis. Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed\u003cbr\u003e\nProduct Citations: Arunachalam PS, et al. 2023. J Clin Invest. 133:. PubMed Dou Y, et al. 2023. Antib Ther. 6:76. PubMed Nunes-Santos CJ, et al. 2023. Nat Commun. 14:3708. PubMed Carre C, et al. 2021. iScience. 24:102970. PubMed Goel RR, et al. 2022. Cell. 185:1875. PubMed Lagou V et al. 2018. Cell reports. 25(3):798-810 . PubMed Sangesland M, et al. 2020. Immunity. 51(4):735-749. PubMed Català-Moll F, et al. 2021. Nucleic Acids Res. 49:5057. PubMed Yang Z, et al. 2018. Front Immunol. 9:2613. PubMed van der Burg M, et al. 2019. Front Immunol. 10:246. PubMed Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed Kohn L, et al. 2014. J Immunol. 192:5050. PubMed Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed Aggarwal C, et al. 2021. J Virol. 95:e0061021. PubMed Flint S, et al. 2016. Haematologica. 101: 698 - 706. PubMed van der Velden V, et al. 2017. J Immunol Methods. 10.1016\/j.jim.2017.03.011. PubMed Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed Rother M, et al. 2016. J Immunol. 197: 441 - 448. PubMed Upasani V, et al. 2019. Front Immunol. 2.152777778. PubMed van Dongen JJM, et al. 2019. Front Immunol. 10:1271. PubMed\u003cbr\u003e\nRRID: AB_961367 (BioLegend Cat. No. 314511) AB_2076098 (BioLegend Cat. No. 314512)\u003cbr\u003e\nStructure: Ig family\u003cbr\u003e\nDistribution: B cells\u003cbr\u003e\nFunction: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nGene ID: 3507\u003cbr\u003e\nUniProt: View information about IgM on UniProt.org\u003cbr\u003e\nClone: MHM-88\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Immunoglobulin M\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866132631721,"sku":"314511","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-314511","provider":"Iright","version":"1.0","type":"link"}