{"product_id":"biolegend-315817","title":"Biolegend, 315817, Brilliant Violet 421™ anti-human CD177 Antibody, 25tests","description":"\u003cp\u003eCD177 is also known as neutrophil specific antigen 1, NB1, and polycythemia rubra vera 1. It is a member of the uPAR family and is a GPI-linked cell surface glycoprotein with a molecular weight of 60 kD. CD177 is expressed on granulocytes and bone marrow progenitors (early erythroblasts, megakaryocytes). It is thought to be involved in allogeneic and autoimmune responses to neutrophils.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human granulocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC – Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting5, and immunofluorescence4.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Leucocyte Typing VII. Mason D, et al. Eds, 2002 Oxford University Press. von Vietinghoff S, et al. 2007. Blood 109:4487. PubMed Korkmaz B, et al. 2008. J. Biol. Chem. 283:35976. PubMed von Vietinghoff S, et al. 2007. Blood 109:4487. (IF) Jankowska AM, et al. 2011. Haematologica. 96:954. (WB)\u003cbr\u003e\nRRID: AB_3683332 (BioLegend Cat. No. 315817) AB_3683332 (BioLegend Cat. No. 315818)\u003cbr\u003e\nStructure: uPAR family, GPI-linked cell surface glycoprotein, 60 kD\u003cbr\u003e\nDistribution: Granulocytes, bone marrow progenitors (early erythroblasts, megakaryocytes)\u003cbr\u003e\nFunction: Antigen involved in neutrophil allo- and autoimmunity, function unknown\u003cbr\u003e\nModification: Glycosylated\u003cbr\u003e\nCell Type: Granulocytes, Hematopoietic stem and progenitors, Neutrophils\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Leukocyte Typing VII. Mason D, et al. (Eds.) Oxford University Press (2002) 2. Kissel K, et al. 2001. Eur. J. Immunol. 31:1301. 3. Lalezari P, et al. 1971. J. Clin. Invest. 50:1108. 4. Temerinac S, et al. 2000. Blood 95:2569.\u003cbr\u003e\nGene ID: 57126\u003cbr\u003e\nUniProt: View information about CD177 on UniProt.org\u003cbr\u003e\nClone: MEM-166\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: Neutrophil specific antigen 1, NB1, polycythemia rubra vera 1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866463129769,"sku":"315817","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-315817","provider":"Iright","version":"1.0","type":"link"}