{"product_id":"biolegend-316312","title":"Biolegend, 316312, APC anti-human β2-microglobulin Antibody, 100tests","description":"\u003cp\u003eβ2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3\/TCR complex of CD8 + T cells.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus, Pig\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Purified human β2-microglobulin\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: Western blotting, and ELISA.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Meissner TB, et al. 2010. Proc Natl Acad Sci USA. PubMed Rizvi SM, et al. 2011. J. Immunol. 186:2309. PubMed Meissner TB, et al. 2012. J Immunol. 188:4951. PubMed.\u003cbr\u003e\nProduct Citations: Chung H et al. 2018. Cell. 172(4):811-824 . PubMed Chimienti R, et al. 2022. Cell Rep. 40:111423. PubMed Sim MJW, et al. 2022. Elife. 11: . PubMed Le TA, et al. 2022. Mol Ther Nucleic Acids. 30:621. PubMed Mitchell K, et al. 2018. J Exp Med. 215:1709. PubMed Zhuang X, et al. 2020. Front Immunol. 2.415972222. PubMed Gondim M, et al. 2015. J Virol. 89: 12518 - 12524. PubMed Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed Kishton RJ, et al. 2022. Cell Rep. 40:111153. PubMed DeWeirdt PC, et al. 2020. Nat Biotechnol. . PubMed Hamilton JR, et al. 2021. Cell Reports. 35(9):109207. PubMed Haideri T, et al. 2022. Cell Rep Methods. 2:100290. PubMed Xu X, et al. 2018. Sci Rep. 8:11649. PubMed Song S, et al. 2021. Front Immunol. 12:705140. PubMed\u003cbr\u003e\nRRID: AB_10643412 (BioLegend Cat. No. 316311) AB_10641281 (BioLegend Cat. No. 316312)\u003cbr\u003e\nStructure: Nonpolymorphic Ig like structure, noncovalently associated with heavy chain of MHC class I molecules, 12 kD\u003cbr\u003e\nDistribution: Leukocytes, platelets, endothelial cells, epithelial cells\u003cbr\u003e\nFunction: Govern MHC class I molecule stability, promote MHC class I molecules binding and presenting peptide antigens to CD8+ T cells\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR complex, CD8\u003cbr\u003e\nCell Type: Endothelial cells, Epithelial cells, Leukocytes, Platelets\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Engelhard VH. 1994. Curr. Opin. Immunol. 6:13. 2. Williams DB, et al. 1989. J. Immunol. 142:2796. 3. Danliczyk UG and TL. Delovitch. 1994. J. Immunol. 153:3533. 4. Williams A, et al. 2002. Tissue Antigens 59:3.\u003cbr\u003e\nGene ID: 567\u003cbr\u003e\nUniProt: View information about beta2-microglobulin on UniProt.org\u003cbr\u003e\nClone: 2M2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: β2M, β2-M, beta2-microglobulin b2-M, b2M\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866449236137,"sku":"316312","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-316312","provider":"Iright","version":"1.0","type":"link"}