{"product_id":"biolegend-317314","title":"Biolegend, 317314, Pacific Blue™ anti-human CD3 Antibody, 100μg","description":"\u003cp\u003eCD3ε is a 20 kD chain of the CD3\/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α\/β or γ\/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin \u0026lt;0.01 EU\/µg).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego. Li B, et al. 2005. Immunology 116:487. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed Alter G, et al. 2008. J. Virol. 82:9668. PubMed Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed Pinto JP, et al. 2010. Immunology. 130:217. PubMed Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed\u003cbr\u003e\nProduct Citations: Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed Beck C, et al. 2023. Biomedicines. 11:. PubMed Côrte-Real BF, et al. 2022. Front Immunol. 13:1005965. PubMed Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed Guo X, et al. 2016. Mol Ther Methods Clin Dev. 3:15054. PubMed Hope C, et al. 2014. Kidney Int. 86:175. PubMed Xia Q, et al. 2022. Methods Mol Biol. 2574:251. PubMed Collins D, et al. 2015. PLoS Pathog. 11: 1005054. PubMed Charles TP, et al. 2021. PLoS Pathog. 17:e1009257. PubMed Bernardes JP, et al. 2020. Immunity. 53:1296. PubMed LaSalle TJ, et al. 2022. Cell Rep Med. 3:100779. PubMed Zaikos TD, et al. 2018. Cell Rep. 25:3759. PubMed Daniels J, et al. 2020. Nat Commun. 11:1806. PubMed Welbourn S, et al. 2022. PLoS Pathog. 18:e1010488. PubMed\u003cbr\u003e\nRRID: AB_571908 (BioLegend Cat. No. 317313) AB_571909 (BioLegend Cat. No. 317314)\u003cbr\u003e\nStructure: Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α\/β or γ\/δ) form the CD3\/TCR complex, 20 kD\u003cbr\u003e\nDistribution: Mature T and NK T cells, thymocyte differentiation\u003cbr\u003e\nFunction: Antigen recognition, signal transduction, T cell activation\u003cbr\u003e\nLigand\/Receptor: Peptide antigen bound to MHC\u003cbr\u003e\nCell Type: NKT cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. Lanier L, et al. 1986. J. Immunol. 137:2501.\u003cbr\u003e\nGene ID: 916\u003cbr\u003e\nUniProt: View information about CD3 on UniProt.org\u003cbr\u003e\nClone: OKT3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: T3, CD3ε\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866157895849,"sku":"317314","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-317314","provider":"Iright","version":"1.0","type":"link"}