{"product_id":"biolegend-317620","title":"Biolegend, 317620, Brilliant Violet 421™ anti-human CD158b1\/j (KIR2DL2\/KIR2DS2) Antibody, 100tests","description":"\u003cp\u003eCD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and possess inhibitory functions and others with short cytoplasmic region lack ITIM and have activation functions. Fifteen polymorphic KIR genes have been reported in humans.  CD158b1 and CD158j, also known as KIR2DL2 and KIR2DS2, respectively, are glycoproteins with two extracellular Ig domains. KIR2DL2 displays a long cytoplasmic tail with ITIM motifs and is 38 kD. KIR2DS2, with a short cytoplasmic tail containing an ITAM motif, is 33 kD in size.  Both KIR2DL2 and KIR2DS2 are expressed primarily on a subset of NK cells and a subset of T cells. KIR2DL2 and KIR2DS2 have been reported to bind to certain HLA-C antigens on a target cell (HLA-C*01, HLA-C*03, HLA-C*07, for example).\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human KIR2DL2 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Clone S22032A does not block the binding of clone DX27 on target cells and is not blocked by DX27. Based on in-house testing on different KIR-expressing transfectants, clone S22032A does not interact with other KIR protein family members.\u003cbr\u003e\nRRID: AB_3720616 (BioLegend Cat. No. 317619) AB_3720616 (BioLegend Cat. No. 317620)\u003cbr\u003e\nStructure: KIR2DL2 is 38 kD and has two extracellular Ig-like domains. KIR2DS2 is 33 kD and has two extracellular Ig-like domains.\u003cbr\u003e\nDistribution: Subset of NK cells, subset of T cells\u003cbr\u003e\nFunction: NK cell regulation\u003cbr\u003e\nLigand\/Receptor: Some HLA-C molecules\u003cbr\u003e\nCell Type: Lymphocytes, NK cells, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Vilches C, et al. 2002. Annu Rev Immunol. 92.107361. Middleton D, et al. 2010. Immunol. 123:8. Wisniewski A, et al. 2012. Human Immu. 73.9. Lin Z, et al. 2016. Cell Reports. 17.9.\u003cbr\u003e\nGene ID: 3803100132285\u003cbr\u003e\nUniProt: View information about CD158b1\/j on UniProt.org\u003cbr\u003e\nClone: S22032A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD158b1 (KIR2DL2, p58.2), CD158j (KIR2DS2, p50.2), KIR-NKAT2\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866277793961,"sku":"317620","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-317620","provider":"Iright","version":"1.0","type":"link"}