{"product_id":"biolegend-317903","title":"Biolegend, 317903, Biotin anti-BrdU Antibody, 25μg","description":"\u003cp\u003eBrdU is a uridine derivative and a structural analog of thymidine that can be incorporated into DNA during the S-phase of the cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval. The MoBU-1 antibody is useful for the detection of incorporated BrdU by fluorescent cell staining (ICFC), flow cytometry, and immunohistochemistry.\u003cbr\u003e\n25μg\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Jung H, et al. 2012. J Exp Med. 209:2409. PubMed\u003cbr\u003e\nProduct Citations: Goldsmith JR, et al. 2020. Nat Commun. 11:2591. PubMed Jung H, et al. 2012. J Exp Med. 209:2409. PubMed\u003cbr\u003e\nRRID: AB_604041 (BioLegend Cat. No. 317904)\u003cbr\u003e\nStructure: Uridine derivative that can be incorporated into DNA and substitute for thymidine residues.\u003cbr\u003e\nDistribution: Cells can be pulsed labeled with BrdU which will be incorporated into DNA during the synthesis phase of the cell cycle.\u003cbr\u003e\nFunction: Antibody against BrdU can be used to identify cells undergoing DNA replication during the period of BrdU incorporation.\u003cbr\u003e\nBiology Area: Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Gratzner HG. 1982. Science 218:474. 2. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573. 3. Dolbeare F, et al. 1985. Cytometry 6:521.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about BrdU on UniProt.org\u003cbr\u003e\nClone: MoBU-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Bromodeoxyuridine\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866464866473,"sku":"317903","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-317903","provider":"Iright","version":"1.0","type":"link"}