{"product_id":"biolegend-320011","title":"Biolegend, 320011, Alexa Fluor® 488 anti-mouse\/rat\/human FOXP3 Antibody, 25tests","description":"\u003cp\u003eFOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4 + \/CD25 - cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in \"scurfy\" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 150D monoclonal antibody reacts with human, mouse and rat FOXP3. The 150D antibody recognizes FOXP3 epitope encoded by exon 2.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nReported Reactivity: Cynomolgus, Rhesus, Baboon\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Full-length FOXP3 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The FOXP3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Roncador G, et al. 2005 Eur. J. Immunol. 35:1681. Mayack. S,et al. 2006. J. Immunol.176:2059. J. Immunol Yang ZZ, et al. 2006. Blood 107:3639. Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659. Groh V, et al. 2006. Nature Immunology 7:755. Lewkowicz P, et al. 2006 J. Immunol. 177:7155. Luke PPW, et al. 2006. Amer. J. Transplant. 6(9):2023. Bamias G, et al. 2007. J. Immunol. 178:1809. Valencia X, et al. 2007. J. Immunol. 178:2579.PubMed Davidson TS, et al. 2007. J. Immunol. 178:4022. MacDonald K PA, et al. 2007. Blood doi:10.1182\/blood-2007-01-067249. Jaffar Z, et al. 2007. J. Immunol. 179:6193. Müller M, et al. 2007. J. Immunol. 179:2774. Jordan JM,et al. 2008.Infect Human. 76:3717. PubMed Golovina TN,et al. 2008. J. Immunol. 181:2855. PubMed Fallarino F, et al. 2009. J. Exp Med. 206:2511. PubMed Banham Alison, et al. 2009. Vet Immunol and Immunop 127.3-4:376-381 Klunker S, et al. 2009. J. Exp Med. PubMed Haque A, et al. 2010. J. Immunol. 184:2583. PubMed Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed\u003cbr\u003e\nProduct Citations: Xie L, et al. 2014. J Immunol. 192:6009. PubMed Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed Chaurio RA, et al. 2022. Immunity. 55:115. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Yu J, et al. 2022. Nat Commun. 13:7903. PubMed Valencia X, et al. 2007. J Immunol . 178:2579. PubMed Li X, et al. 2019. Mol Med Rep. 19:4195. PubMed Li Q, et al. 2022. Cell Rep. 40:111308. PubMed Zhu P, et al. 2012. J Cereb Blood Flow Metab. 0.482638889. PubMed Berg S 2006. J Immunol. 176:2059. PubMed Gkountidi AO, et al. 2021. Cancer Immunol Res. 9:748. PubMed Xiong Y, et al. 2012. J Biol Chem. 287:34372. PubMed Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed Renga G, et al. 2020. Life Sci Alliance. 3:. PubMed Meng KP, et al. 2020. J Exp Med. 217:00:00. PubMed Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed Runge EM, et al. 2020. J Neuroinflammation. 17:121. PubMed Perkins T, et al. 2017. J Allergy Clin Immunol Pract. 10.1016\/j.jaip.2017.01.028. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed An J, et al. 2022. iScience. 25:103570. PubMed PRADO-GARCIA H, et al. 2015. Anticancer Res. 35:1529. PubMed Shi R, et al. 2022. Theranostics. 12:875. PubMed Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed Becher J, et al. 2018. Dev Cell. 47:592. PubMed Cimbro R, et al. 2014. Proc Natl Acad Sci U S A. 6:81. PubMed Liu B, et al. 2022. Mol Med Rep. 26:. PubMed Murakami R, et al. 2013. PLoS One. 8:73270. PubMed Oida T, Weiner H 2010. PLoS One. 5:e15523. PubMed Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed Martínez–Fábregas J, et al. 2019. Elife. 8:e49314. PubMed Zhu D, et al. 2017. Stem Cell Res Ther. 0.511805556. PubMed Luck H, et al. 2019. Nat Commun. 10:3650. PubMed Machhi J, et al. 2021. J Neuroinflammation. 18:272. PubMed Dotsey E, et al. 2017. Sci Rep. 7:42584. PubMed Nakazawa Y, et al. 2021. Elife. 10:. PubMed Groen B, et al. 2015. Sci Rep. 5: 13618. PubMed Chmielewski M and Abken H 2017. Cell Rep.. 10.1016\/j.celrep.2017.11.063. PubMed Su M, et al. 2018. Immunol Cell Biol. 9:2448. PubMed Pflügler S, et al. 2020. Commun Biol. 3:252. PubMed Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed Golovina T, et al. 2008. J Immunol. 181:2855. PubMed Nesterenko PA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed\u003cbr\u003e\nRRID: AB_439747 (BioLegend Cat. No. 320011) AB_439748 (BioLegend Cat. No. 320012)\u003cbr\u003e\nStructure: Forkhead\/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains\u003cbr\u003e\nDistribution: Nuclear; expressed in T regulatory cells\u003cbr\u003e\nFunction: Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis\u003cbr\u003e\nInteraction: Interacts with DNA\u003cbr\u003e\nCell Type: Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Hori S, et al. 2003. Science 299:1057.\u003cbr\u003e\nRegulation: FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation\u003cbr\u003e\nGene ID: 2037131738250943\u003cbr\u003e\nUniProt: View information about FOXP3 on UniProt.org\u003cbr\u003e\nClone: 150D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?\u003cbr\u003e\nA: It is not recommended. It is best to use PBMCs for this testing.\u003cbr\u003e\nQ: Can FOXP3 be costained with cytokines?\u003cbr\u003e\nA: The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860811272361,"sku":"320011","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-320011","provider":"Iright","version":"1.0","type":"link"}